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Cell-size distribution of human tonsillar lymphocytes

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Summary

Cell-size distributions of human tonsillar lymphocytes (TL) and adenoid lymphocytes (AL) were determined using a computerized cell-size analyser. In comparison with peripheral blood lymphocytes, TL, and AL comprised a greater number of large lymphocytes. Child TL were characterized by an enrichment of not only B-lymphocytes but also of large lymphocytes. A decrease in the number of large B-lymphocytes with advancing age was considered to be indicative of the morphological profile of tonsillar involution.

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References

  1. Bianco C, Patrick R, Nussenzweig V (1970) A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med 132: 702–720

    Google Scholar 

  2. Brochier J, Samarut C, Bich-Thuy LT, Revillard JP (1978) Study of human T and B lymphocytes with heterologous antisera. IV. Subpopulations in tonsil and adenoid cell suspensions. Immunol 35: 827–835

    Google Scholar 

  3. Coulter WH (1953) Means for counting particles suspended in a fluid. US Patent 2,656,508

  4. Elliott BE (1979) A sensitive method for the separation of rosette-forming cells. In: Lefkovits I, Pernis B (eds) Immunological methods. Academic Press, New York, pp 241–259

    Google Scholar 

  5. Gregg EC, Steidley KD (1965) Electrical counting and sizing of mammalian cells in suspension. Biophys J 5: 393–405

    Google Scholar 

  6. Gyeney L, Merétey K, Falus A, Böhm U, Bozsóky S (1978) Clinical immunological study of T lymphocytes and EA rosette forming cells in tonsils. Arch Otorhinolaryngol 221: 105–113

    Google Scholar 

  7. Julius MH, Simpson E, Herzenberg LA (1973) A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol 3: 645–649

    Google Scholar 

  8. Kobayashi K, Kanoh H (1981) Incorporation of radioactive free fatty acids into glycerolipids of subcelullar fractions from human tonsil lymphocytes. Tohoku J Exp Med 133: 105–118

    Google Scholar 

  9. Nowell PC (1960) Phytohemagglutinin: an initiator of mitosis in cultures of normal human leucocytes. Cancer Res 20: 462–466

    Google Scholar 

  10. Oettgen HF, Silber R, Miescher PA, Hirschhorn K (1966) Stimulation of human tonsillar lymphocytes in vitro. Clin exp Immunol 1: 77–84

    Google Scholar 

  11. Pennline KJ (1981) Separation of macrophages-enrichment: adherence to plastic or glass surfaces. In: Herscowitz HB, Holden HT, Bellanti JA, Ghaffer A (eds) Manual of macrophage methodology. Marcel Dekker, New York Basel, pp 63–68

    Google Scholar 

  12. Schwarz MR (1967) Response of thymus and other human lymphoid tissues to PHA, PWM and genetically dissimilar lymphoid cells. Proc Soc Exp Biol Med 125: 701–705

    Google Scholar 

  13. Siegel G (1979) Evidence for selective proliferation and histological localisation of B lymphocytes in the human tonsil. ORL 41: 147–157

    Google Scholar 

  14. Siegel G (1979) Biochemical characterization of the proliferation pool of lymphatic cells in the human tonsil and demonstration of the age-dependent pool size reduction. Acta Otolaryngol 87: 560–566

    Google Scholar 

  15. Siegel G (1980) Quantitative Veränderungen von B- und T-Lymphozyten während der Tonsilleninvolution. HNO-Praxis 5: 283–294

    Google Scholar 

  16. Staub M, Antoni F, Sellyei M (1976) DNA snythesis in tonsil lymphocytes. I. Changes in cell population during culture. Biochem Med 15: 246–253

    Google Scholar 

  17. Watanabe T, Yashizaki K, Yagura T, Yamamura Y (1974) In vitro antibody formation by human tonsil lymphocytes. J Immunol 113: 608–616

    Google Scholar 

  18. Wybran J, Chantler S, Fudenberg HH (1973) Human blood T cells: response to phytohe-magglutinin. J Immunol 110: 1157–1160

    Google Scholar 

  19. Yamanaka N, Kobayashi K, Himi T, Shido F, Kataura A (1983) Spontaneous DNA synthesis in tonsillar lymphocytes and its clinical implications. Acta Otolaryngol 96: 181–187

    Google Scholar 

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Shido, F., Kobayashi, K. & Yamanaka, N. Cell-size distribution of human tonsillar lymphocytes. Arch Otorhinolaryngol 239, 211–218 (1984). https://doi.org/10.1007/BF00464246

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  • DOI: https://doi.org/10.1007/BF00464246

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