Summary
Fibronectin was localized in human cholesteatoma tissues by immunohistochemical methods. The avidin-biotin-peroxidase complex staining method was used with specific fibronectin antibody. Fibronectin appeared to be localized in the matrix of the cholesteatoma studied, particularly on the surface of the cell membranes and the nuclei of the basal cells and in connective tissue. Fibronectin was not seen in the granular layer or in the keratin area. Fibronectin was found on the surface of granulation tissue, mononuclear cells, fibroblasts and endothelial cells of blood vessels. These findings were confirmed by the immunofluorescent staining method. Our previous study showed that fibronectin induced a migration of keratinocytes, macrophages and fibroblasts demonstrated by the Boyden's chamber chemotaxis assay. Macrophages and fibroblasts were shown to produce collagenase, a bone resorption factor, in cholesteatomatous tissue. The present study showed the presence of fibronectin in the matrix of cholesteatoma and granulation tissue, suggesting that fibronectin might play an important role in the clinical development and invasive behavior of cholesteatoma.
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References
Abramson M, Huang CC (1977) Localization of collagenase in human middle ear cholesteatoma. Laryngoscope 87:771–791
Abramson M, Gantz BJ, Asarch RG, Litton WB (1977) Cholesteatoma pathogenesis: evidence for the migration theory. In: McCabe BF, Sade J, Abramson M (eds) Cholesteatoma First International Conference. Aesculapius, Birmingham, pp 176–186
Boselli JM, Macarak EJ, Clark CC, Brownell AG, Martinez-Hernandez A (1981) Fibronectin: its relationship to basement membranes. I. Light microscopic studies. Coll Relat Res 5:391–404
Clark RAF, Lanigan JM, DellaPelle P, Manseau E, Dvorak HF, Colvin RB (1982) Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelization. J Invest Dermatol 79:264–269
Couchman JR, Gibson WT, Thom D, Weaver AC, Rees DA, Parish WE (1979) Fibronectin distribution in epithelial and associated tissues of the rat. Arch Dermatol Res 266:295–310
Cutolo M, Accardo S, Zardi L (1985) Different fibronectin concentrations in plasma samples drawn from the affected and unaffected arms of a pagetic patient. J Rheumatol 12:176–178
Fyrand O (1979) Studies on fibronectin in the skin. II. Indirect immunofluorescence studies in psoriasis vulgaris. Arch Dermatol Res 266:33–41
Fyrand O (1980) Studies on fibronectin in the skin. IV. Indirect immunofluorescence studies in lupus erythematosus. Br J Dermatol 102:167–171
Huang CC (1987) Bone resorption in experimental otosclerosis in rats. Am J Otolaryngol 8:332–341
Kubo M, Norris DA, Howell SE, Ryan SR, Clark RAF (1984) Human keratinocytes synthesize, secrete, and deposit fibronectin in the pericellular matrix. J Invest Dermatol 82:580–586
Kuusela P, Ruoslahti E, Engvall E, Vaheri A (1976) Immunological interspecies cross-reactions of fibroblast surface antigen (fibronectin). Immunochemistry 13:639–642
Labat-Robert J, Robert L, Auger C, Lethias C, Carrone R (1981) Fibronectin-like protein in porifera: its role in cell aggregation. Proc Natl Acad Sci USA 78:6261–6265
Moriyama H, Huang CC, Abramson M, Kato M (1984) Bone resorption factors in chronic otitis media. Otolaryngol Head Neck Surg 92:322–328
Morrison PR, Edsall JT, Miller SG (1948) Preparation and properties of serum and plasma proteins. XVIII. The separation of purified fibrinogen from fraction I of human plasma. J Am Chem Soc 70:3103–3108
Nagata H, Ueki H, Moriguchi T (1985) Fibronectin. Localization in normal human skin, granulation tissue, hypertrophic scar, mature scar, progressive systemic sclerotic skin, and other fibrosing dermatoses. Arch Dermatol 121:995–999
O'Keefe EJ, Woodley D, Castillo G, Russell N, Payne RE Jr (1984) Production of soluble and cell-associated fibronectin by cultured keratinocytes. J Invest Dermatol 82: 150–155
O'Keefe EJ, Payne REJr, Russell N, Woodley DT (1985) Spreading and enhanced motility of human keratinocytes on fibronectin. J Invest Dermatol 85:125–130
Proctor RA, Textor JA, Vann JM, Mosher DF (1985) Role of fibronectin in human monocyte and macrophage bactericidal activity. Infect Immun 47:629–637
Ruoslahti E, Vaheri A (1975) Interaction of soluble fibroblast surface antigen with fibrinogen and fibrin. Identity with cold insoluble globulin of human plasma. J Exp Med 141:497–501
Ruoslahti E, Vaheri A, Kuusela P, Linder E (1973) Fibroblast surface antigen: a new serum protein. Biochim Biophys Acta 322:352–358
Stenman S, Vaheri A (1978) Distribution of a major connective tissue protein, fibronectin, in normal human tissue. J Exp Med 147:1054–1064
Sugita T, Huang CC, Abramson M (1987) The effect of fibronectin on the migration of keratinocytes. Acta Otolaryngol (Stockh) 104:521–525
Takashima A, Grinnell F (1984) Human keratinocyte adhesion and phagocytosis promoted by fibronectin. J Invest Dermatol 83:352–358
Toda K, Grinnell F (1987) Activation of human keratinocyte fibronectin receptor function in relation to other ligand-receptor interactions. J Invest Dermatol 88:412–417
Yamada KM, Olden K (1978) Fibronectins — adhesive glycoproteins of cell surface and blood. Nature 275:179–184
Zardi L, Cecconi C, Barbieri O, Carnemolla B, Picca M, Santi L (1979) Concentration of fibronectin in plasma of tumor-bearing mice and synthesis by Ehrlich ascites tumor cells. Cancer Res 39:3774–3779
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Chao, W.Y., Yuan, Q.G. & Huang, C.C. Localization of fibronectin in human middle ear cholesteatoma. Arch Otorhinolaryngol 245, 160–165 (1988). https://doi.org/10.1007/BF00464019
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DOI: https://doi.org/10.1007/BF00464019