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Diabetologia

, Volume 9, Issue 6, pp 499–504 | Cite as

Action of insulin and glucose on the re-esterification rate of free fatty acids in isolated human fat cells

  • H. -J. Lisch
  • S. Sailer
  • F. Sandhofer
  • W. Schennach
  • H. Braunsteiner
Originals

Summary

In isolated human fat cells of the greater omentum and the mammary gland, the effect of glucose, fructose, and/or insulin was tested on the re-esterification rate of FFA measured by the balance method. It could be shown that in the absence of glucose no re-esterification activity was demonstrable. Glucose alone or fructose alone stimulated the re-esterification of FFA dose-dependently in isolated fat cells of the greater omentum, and to a minor degree in fat cells of the mammary gland. Insulin had no effect on the re-esterification rate of FFA in the presence or absence of glucose or fructose, whereas it significantly stimulated the incorporation of glucose-C into CO2 and lipids. It is concluded that the re-esterification of FFA in human adipose tissue, at least in vitro, is mainly controlled by glucose without need for insulin.

Key words Glucose fructose insulin isolated fat cells re-esterification of free fatty acids 

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • H. -J. Lisch
    • 1
  • S. Sailer
    • 1
  • F. Sandhofer
    • 1
  • W. Schennach
    • 1
  • H. Braunsteiner
    • 1
  1. 1.Medizinische Universitätsklinik and Chirurgische UniversitätsklinikInnsbruckAustria

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