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Effect of generalized graft-versus-host-reaction on B- and T-lymphocytes and a benzpyrene-induced murine sarcoma

  • Ch. Witting
  • E. Hultsch
Original Works

Summary

The investigations of 400 adult hybrids from A/Jax fem. BALB/c male were aimed at answering the following questions:
  1. 1.

    What is the influence of a generalized graft-versus-host-reaction (GVHR) on the induction and growth of benzpyrene-induced sarcoma?

     
  2. 2.

    What is the behavior of B- and T-lymphocytes during GVHR, and during induction and growth of benzpyrene-induced sarcoma?

     
  3. 3.

    Which are the deductions to be drawn with regard to the effect of B- and T-lymphocytes during tumorigenesis and tumor growth?

     

Results

  1. 1.

    The number of tumors was significantly greater in the GVHR animals (74.5%) than in the controls (49.5%).

     
  2. 2.

    A significant decrease of T-lymphocytes and increase of B-lymphocytes during GVHR were found in the peripheral blood.

     
  3. 3.

    The examination of the tumor marginal zone showed a marked decrease of the total round cell count and the T cells, while the count of B-lymphocytes remained uninfluenced.

     

These results are discussed and compared with results of other authors.

Key words

Graft-versus-host-reaction Benzpyrene sarcoma B- and T-lymphocytes 

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References

  1. Argyris, B.F.: The role of macrophages, thymus, and bone marrow-derived cells in the graft-versushost reaction. Transplantation 17, 387–391 (1974)Google Scholar
  2. Bach, J.F., Gigli, J., Dardenne, M., Dormont, J.: Mechanism of complement action in rosette inhibition and lymphocytotoxicity of antilymphocyte serum. Immunology 22, 625–635 (1972)Google Scholar
  3. Blomgren, H., Jacobsson, H.: Interaction between thymocytes and lymph-node cells in the graft versus-host response: evidence for a synergistic and an antagonistic activity mediated by mouse thymocytes. Cell. Immunol. 12, 296–308 (1974)Google Scholar
  4. Böyum, A.: Isolation of leukocytes from human blood. Scand. J. clin. lab. Invest. 21 (Suppl. 98), 9–89 (1968)Google Scholar
  5. Danielson, J.R., Alten, P.J. van: Lymphocyte proliferation inhibited by cells and by effector substances obtained from bursal lymphocytes. Tumor Res. 19, 194–202 (1974)Google Scholar
  6. Elkins, W.L.: Cellular immunology and the pathogenesis of graft-versus-host-reactions. Progr. Allergy 15, 78–187 (1971)Google Scholar
  7. Gleichmann, E., Gleichmann, H.: Graft-versus-host reaction: a pathogenic principle for the development of drug allergy, autoimmunity, and malignant lymphoma in non-chimeric individuals. Hypothesis. Z. Krebsforsch. 85, 91–109 (1976)Google Scholar
  8. Hagmann, J., Hess, M.W., Keller, H.U., Cottier, H.: Cell systems participating in graft rejections. In: Masshoff, J.W. (Ed.) Transplantation. Handbuch der allgemeinen Pathologie, Band 6, Teil 8. Berlin, Heidelberg, New York: Springer 1977Google Scholar
  9. Hobik, H.P.: Graft-versus-host-reactions. In: Masshoff, J.W. (Ed.) Transplantation. Handbuch der allgemeinen Pathologie, Band 6, Teil 8. Berlin, Heidelberg, New York: Springer 1977Google Scholar
  10. Jondal, M., Holm, G., Wigzell, H.: Surface markers on human T-and B-lymphocytes I. A large population of lymphocytes forming non-immune rosettes with sheep red blood cells. J. exp. Med. 136, 207–215 (1972)Google Scholar
  11. Lennert, K., Rinneberg, H.: Fermenthistochemische Untersuchungen des Lymphknotens II. Mitteilung: Adenosintriphosphatase und 5′-Nucleotidase im Lymphknotenschnitt. Klin. Wschr. 39, 923–924 (1961)Google Scholar
  12. Müller, J., Keller, H.U., Brun del Re, G., Buerki, H., Hess, M.W.: Nonspecific esterase activity in T cells. In: Feldman, M., Globerson, A. (Ed.): Immune reactivity of lymphocytes, Plenum Publishing corporation 1976Google Scholar
  13. Müller-Hermelink, H.K.: Characterization of the B-cell regions of human lymphatic tissues through enzyme histochemical demonstration of ATP-ase and 5′-Nucleotidase activities. Virchows Arch. B Cell Path. 16, 371–378 (1974)Google Scholar
  14. Nie, N.N., Hull, C.H., Jenkins, J.G., Steinbrenner, K., Bent, D.H.: Statistical Package for the Social Sciences (SPSS) McGraw-Hill Book Company, Second Edition 1975Google Scholar
  15. Niedorf, H.R.: Das lymphatische Gewebe des Hühnchens nach Bursektomie und bei experimenteller Onkogenese. Habilitationsschrift der Westfälischen Wilhelms-Universität Münster 1975Google Scholar
  16. Okubo, S., Oettgen, H.F., Calazza, S.S., Ovary, Z.: Enhancement of tumor growth by graft-versushost reaction. Proc. Nat. Acad. Sci. 71, 4264–4266 (1974)Google Scholar
  17. Radaszkiewicz, T., Denk, H.: Identification of thymus-dependent areas in frozen sections of guinea pig lymphatic tissue by adherence of rabbit erythrocytes. Cell. Immunol. 14, 303–306 (1974)Google Scholar
  18. Raff, M.C., Owen, J.J.T.: Thymus-derived lymphocytes: their distribution and role in the development of peripheral lymphoid tissues of the mouse. Eur. J. Immunol. 1, 27–30 (1971)Google Scholar
  19. Schröder, J., de la Chapelle, A.: Fetal lymphocytes in the maternal blood. Blood 39, 153–162 (1972)Google Scholar
  20. Seiler, F.R., Sedlacek, H.H., Kanzy, E.J., Lang, W.: Über die Brauchbarkeit immunologischer Nachweismethoden zur Differenzierung funktionell verschiedener Lymphozyten: Spontanrosetten, Komplementrezeptor-Rosetten und Immunglobulinrezeptoren. Behring Inst. Mitt. 52, 26–72 (1972)Google Scholar
  21. Silveira, N.P.A., Mendes, N.F., Tolnai, M.E.A.: Tissue localization of two populations of human lymphocytes. Distinguished by membrane receptors. J. Immunol. 108, 1456–1460 (1972)Google Scholar
  22. Solnik, C., Gleichmann, H., Kavanah, M., Schwartz, R.S.: Immunosuppression and malignant lymphomas in graft-versus-host reactions. Cancer Res. 33, 2068–2077 (1973)Google Scholar
  23. Trepel, F.: Das lymphatische Zellsystem: Struktur, allgemeine Physiologie und allgemeine Pathophysiologie. In: Schwiegk, H. (Ed.): Handbuch der inneren Medizin II/3. Berlin, Heidelberg, New York: Springer 1976Google Scholar
  24. Uhr, J.W.: Passive sensitization of lymphocytes and macrophages by antigen-antibody complexes. Proc. Nat. Acad. Sci. 54, 1599–1606 (1965)Google Scholar
  25. Warner, N.L., Szenberg, A.: Immunologic studies on hormonally bursectomized and surgically thymectomized chickens: Dissociation of immunologic responsiveness. In: Good, R.A., Gabrielsen, A.E. (Eds.): The Thymus in Immunobiology, Hoeber Medical Division, New York, Evanston, London 1964Google Scholar
  26. Witting, Ch., Detlefsen, W.: Zur Korrelation von Splenomegalie und Blutbildveränderungen bei der graft-versus-host-Reaktion der Maus. Blut 31, 229–238 (1975)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Ch. Witting
    • 1
    • 2
  • E. Hultsch
    • 1
    • 2
  1. 1.Pathologisches Institut der UniversitätMünsterFederal Republic of Germany
  2. 2.Institut für Medizinische Informatik und Biomathematikrt der UniversitätMünsterFederal Republic of Germany

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