Archives of oto-rhino-laryngology

, Volume 206, Issue 2, pp 127–136 | Cite as

Passive transport of the stria vascularis

  • Y. Nakai
Article

Summary

The transport and diffusion of the stria vascularis were studied by injection of ferritin and horseradish peroxidase into the endolymphatic space of the guinea pig. The observation was made by electron microscopy under the conditions with or without the apposition of NaCl crystals on the round window membrane.

In the cochlea with no NaCl crystals on the round window membrane, ferritin or HRP was found only in the apical cytoplasm of the marginal cells. No trace of particles was seen in the intermediate, basal, endothelial cells and intercellular spaces in the stria vascularis.

The ferritin passed through the marginal cells, intercellular spaces, basement membrane and capillary walls by vesicular transport under the condition of change of osmotic pressure by apposition of NaCl crystals on the round window membrane.

HRP labeled the marginal cells but was not seen in the intermediate, basal, endothelial cells and intercellular spaces.

The functional significance of these findings were discussed.

Keywords

Ferritin Osmotic Pressure Intercellular Space Capillary Wall Vesicular Transport 

Zusammenfassung

Transport und Diffusion der Stria vascularis wurden durch Injektion von Ferritin und Meerrettich-peroxidase (MRP) in den Endolymphraum des Meerschweinchens untersucht und die Veränderungen elektronenmikroskopisch dargestellt. Einem Teil der Tiere wurden Kochsalzkristalle auf die Membran des runden Fensters appliziert. Bei den Schnecken ohne Kochsalzkristalle am runden Fenster wurde Ferritin oder MRP nur im apikalen Teil des Cytoplasmas der Marginalzellen gefunden. Die intermediären, die basalen und die Endothelzellen sowie die intercellulären Räume der Stria vascularis waren frei.

Bei Änderung des osmotischen Druckes nach Einlage von Kochsalzkristallen auf die runde Fenstermembran passiert Ferritin die Marginalzellen, die intercellulären Räume, die Basalmembran und die Capillarwände mittels vesiculären Transports. MRP fand sich in den Marginalzellen, nicht aber in den intermediären, basalen und endothelialen Zellen und den intercellulären Räumen. Die funktionelle Bedeutung dieser Befunde wird diskutiert.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Y. Nakai
    • 1
  1. 1.Department of Otolaryngology Osaka City University Medical SchoolOsakaJapan

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