Skip to main content

Sulfate assimilation in Rhodopseudomonas globiformis

Abstract

Rhodopseudomonas globiformis is able to grow on sulfate as sole source of sulfur, but only at concentrations below 1 mM. Good growth was observed with thiosulfate, cysteine or methionine as sulfur sources. Tetrathionate supported slow growth. Sulfide and sulfite were growth inhibitory. Growth inhibition by higher sulfate concentrations was overcome by the addition of O-acetylserine, which is known as derepressor of sulfate-assimilating enzymes, and by reduced glutathione. All enzymes of the sulfate assimilation pathway. ATP-sulfurylase, adenylylphosphate-sulfotransferase, thiosulfonate reductase and O-acetylserine sulfhydrylase are present in R. globiformis. Sulfate was taken up by the cells and the sulfur incorporated into the amino acids cysteine, methionine and homocysteine. It is concluded, that the failure of R. globiformis to grow on higher concentrations of sulfate is caused by disregulation of the sulfate assimilation pathway. Some preliminary evidence for this view is given in comparing the activities of some of the involved enzymes after growth on different sulfur sources and by examining the effect of O-acetylserine on these activities.

This is a preview of subscription content, access via your institution.

Abbreviations

DTE:

dl-dithioerythritol

APS:

adenosine 5′-phosphosulfate, adenylyl sulfate

PAPS:

3′-phosphoadenosine 5′-phosphosulfate, 3′-phosphoadenylylsulfate

References

  • Haworth C, Heathcote JG (1969) An improved technique for the analysis of amino acids and related compounds on thin layers of cellulose. J Chromatogr 41:380–385

    Google Scholar 

  • Hensel G, Trüper HG (1976) Cysteine and S-sulfocysteine biosynthesis in phototrophic bacteria. Arch Microbiol 109: 101–103

    Google Scholar 

  • Imhoff JF (1980) Aspekte des assimilatorischen Schwefelstoffwechsels in Rhodospirillaceae. Doctoral Thesis, University of Bonn, Germany

    Google Scholar 

  • Jones-Mortimer MC, Wheldrake JF, Pasternak CA (1968) The control of sulphate reduction in E. coli by O-acetyl-l-serine. Biochem J 107:51–53

    Google Scholar 

  • Lowry OH, Rosebrough HJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Moore ST (1963) On determination of cysteine as cysteic acid. J Biol Chem 238:235–237

    Google Scholar 

  • Novozamsky I, van Eck R (1977) Total sulfur determination in plant material. Z Anal Chem 286:367–368

    Google Scholar 

  • Pfennig N (1974) Rhodopseudomonas globiformis, sp. n., a new species of the Rhodospirillaceae. Arch Microbiol 100:197–206

    Google Scholar 

  • Schmidt A (1972) On the mechanism of photosynthetic sulfate reduction. An APS-sulfotransferase from Chlorella. Arch Mikrobiol 84:77–86

    Google Scholar 

  • Schmidt A (1973) Sulfate reduction in a cell-free system of Chlorella. The ferredoxin dependent reduction of a protein-bound intermediate by a thiosulfonate reductase. Arch Mikrobiol 93:29–52

    Google Scholar 

  • Spackman DH, Stein WH, Moore ST (1958) Automatic recording apparatus for use in the chromatography of amino acids. Anal Chem 30:1190–1206

    Google Scholar 

  • Then J, Trüper HG (1981) The role of thiosulfate in sulfur metabolism of Rhodopseudomonas globiformis. Arch Microbiol 130:143–146

    Google Scholar 

  • Urban PJ (1961) Colorimetry of sulfur anions. I. An improved colorimetric method for the determination of thiosulfate. Z Anal Chem 179:415–426

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Imhoff, J.F., Then, J., Hashwa, F. et al. Sulfate assimilation in Rhodopseudomonas globiformis . Arch. Microbiol. 130, 234–237 (1981). https://doi.org/10.1007/BF00459525

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00459525

Key words

  • Rhodospirillaceae
  • Rhodopseudomonas globiformis
  • Sulfate assimilation