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Archives of oto-rhino-laryngology

, Volume 244, Issue 2, pp 88–90 | Cite as

Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube

  • Y. Ohashi
  • Y. Nakai
  • H. Ikeoka
  • H. Koshimo
  • Y. Esaki
  • S. Kato
Article

Summary

We have used a tissue culture technique and a photoelectric method to examine the direct effect of lipopolysaccharide (LPS) on the ciliary activity present in the eustachian tube. Since LPS possesses the major part of the biological activity of endotoxin, our results show clearly that LPS deteriorates the ciliary activity in a dose-response fashion: LPS does not deteriorate the ciliary activity up to 168 h if its concentration is 1ng/ml or less; 10 ng/ml LPS can cause deterioration of the ciliary activity with extended exposure (more than 96 h); LPS can cause dysfunction of the cilia rather quickly if the concentration is 100 ng/ml or more. Our results show that the ciliary activity in the eustachian tube under clinical conditions can be affected by endotoxin.

Key words

Ciliary activity Otitis media with effusion Lipopolysaccharide Eustachian tube 

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References

  1. 1.
    Bernstein JM, Praino MD, Neter E (1980) Detection of endotoxin in ear specimens from patients with chronic otitis media by means of limulus amebocyte test. Can J Microbiol 26:645–648Google Scholar
  2. 2.
    Bluestone CD, Berry QC (1976) Concept on the pathogenesis of middle ear effusion. Ann Otol Rhinol Laryngol [Suppl 25] 85:182–186Google Scholar
  3. 3.
    DeMaria TF, Briggs BR, Okazaki N, Lim DJ (1984) Experimental otitis media with effusion following middle ear inoculation of nonviable H. influenzae. Ann Otol Rhinol Laryngol 93:52–56Google Scholar
  4. 4.
    DeMaria TF, Prior RB, Briggs BR, Lim DJ, Herbert G (1984) Endotoxin in middle-ear effusion with chronic otitis media with effusion. J Clin Microbiol 20:15–17Google Scholar
  5. 5.
    Healy BG, Teele DW (1977) The microbiology of chronic middle ear effusions in children. Laryngoscope 87:1472–1478Google Scholar
  6. 6.
    Holmgren L (1940) Experimental tubal occlusion. Acta Otolaryngol (Stockh) 28:587–592Google Scholar
  7. 7.
    Iino Y, Yuasa R, Kaneko Y, Takasaka T (1985) Endotoxin in the middle ear effusion: in case with persistent effusion after acute otitis media. Auris Nasus Larynx (Tokyo) [Suppl 1] 12:94–96Google Scholar
  8. 8.
    Jinnin T, Dake Y, Kusumoto T, Tabata T (1985) Quantitative determination of bacterial endotoxin in middle ear effusion by chromogenic substrate method. Auris Nasus Larynx (Tokyo) [Suppl 1] 12:97–99Google Scholar
  9. 9.
    Jordan R (1949) Chronic otitis media. Laryngoscope 59:1002–1015Google Scholar
  10. 10.
    Kihara S, Ohashi Y, Maruoka K, Ikeoka H, Masutani H, Nakai Y (1985) The ciliary activity of the middle ear lining in some pathological states. Auris Nasus Larynx (Tokyo) [Suppl 12] 12:126–128Google Scholar
  11. 11.
    Lim DJ, DeMaria TF (1982) Pathogenesis of otitis media: bacteriology and immunology. Laryngoscope 92:278–286Google Scholar
  12. 12.
    Liu YS, Lim DJ, Lang RW (1975) Chronic middle ear effusions: immunochemical and bacteriological investigation. Acta Otolaryngol (Stockh) 101:278–286Google Scholar
  13. 13.
    Moore GE, Gerner RE, Flanklin HA (1967) Culture of normal human leukocytes. JAMA 199:519–524Google Scholar
  14. 14.
    Nonomura N, Nakano Y, Satoh Y, Fujioka O, Niijima H, Fujita M (1986) Otitis media with effusion following inoculation of Haemophilus influenzae type b endotoxin. Arch Otorhinolaryngol 243:31–35Google Scholar
  15. 15.
    Ohashi Y, Nakai Y (1983) Functional and morphological studies on chronic sinusitis mucous membrane. 1. Decline of ciliary action in chronic sinusitis. Acta Otolaryngol (Stockh) [Suppl] 397:3–8Google Scholar
  16. 16.
    Ohashi Y, Nakai Y (1984) Mucociliary activities in fetal rabbits. Acta Otolaryngol (Stockh) 97:351–358Google Scholar
  17. 17.
    Ohashi Y, Nakai Y, Kihara S (1985) Ciliary activity of the middle ear lining in guinea pigs. Ann Otol Rhinol Laryngol 94:419–423Google Scholar
  18. 18.
    Ohashi Y, Nakai Y, Kihara S, Maruoka K, Ikeoka H, Uemura Y (1985) The ciliary activity of the middle ear lining: functional and morphological observation. Auris Nasus Larynx (Tokyo) [Suppl 1] 12:123–125Google Scholar
  19. 19.
    Ohashi Y, Nakai Y, Ikeoka H, Koshimo H, Onoyama Y (1985) Effects of irradiation on the ciliary activity of the eustachian tube and the middle ear mucosa. Arch Otorhinolaryngol 242:343–348Google Scholar
  20. 20.
    Ohashi Y, Nakai Y, Ikeoka H, Koshimo H, Esaki Y (1986) Mucosal pathology of the eustachian tube after exposure to sulfur dioxide. Arch Otorhinolaryngol 243:274–279Google Scholar
  21. 21.
    Ohashi Y, Nakai Y, Koshimo H, Esaki Y (1986) Ciliary activity in the in vitro tubotympanum. Arch Otorhinolaryngol 243:317–319Google Scholar
  22. 22.
    Ohashi Y, Nakai Y, Kihara S, Ikeoka H (1987) Effects of S. aureus on the ciliary activity of the middle ear lining. Ann Otol Rhinol Laryngol (in press)Google Scholar
  23. 23.
    Ohashi Y, Nakai Y, Ikeoka H, Koshimo H, Esaki Y, Horiguchi S, Teramoto K, Nakaseko H (1987) An experimental study on the acute effects of isopropyl alcohol exposure on the middle ear mucosa. J Appl Toxicol (in press)Google Scholar
  24. 24.
    Senturia BH, Gessert DF, Carr CD (1958) Studies concerned with tubotympanitis. Ann Otol Rhinol Laryngol 67:440–467Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Y. Ohashi
    • 1
  • Y. Nakai
    • 1
  • H. Ikeoka
    • 1
  • H. Koshimo
    • 1
  • Y. Esaki
    • 1
  • S. Kato
    • 1
  1. 1.Department of OtolaryngologyOsaka City University Medical SchoolOsakaJapan

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