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Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube

Archives of oto-rhino-laryngology volume 244, pages 88–90 (1987)Cite this article

Summary

We have used a tissue culture technique and a photoelectric method to examine the direct effect of lipopolysaccharide (LPS) on the ciliary activity present in the eustachian tube. Since LPS possesses the major part of the biological activity of endotoxin, our results show clearly that LPS deteriorates the ciliary activity in a dose-response fashion: LPS does not deteriorate the ciliary activity up to 168 h if its concentration is 1ng/ml or less; 10 ng/ml LPS can cause deterioration of the ciliary activity with extended exposure (more than 96 h); LPS can cause dysfunction of the cilia rather quickly if the concentration is 100 ng/ml or more. Our results show that the ciliary activity in the eustachian tube under clinical conditions can be affected by endotoxin.

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Affiliations

  1. Department of Otolaryngology, Osaka City University Medical School, 1-5-7 Asahimachi, Abenoku, 545, Osaka, Japan

    Y. Ohashi, Y. Nakai, H. Ikeoka, H. Koshimo, Y. Esaki & S. Kato

Authors
  1. Y. Ohashi
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  2. Y. Nakai
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  3. H. Ikeoka
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  4. H. Koshimo
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  5. Y. Esaki
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  6. S. Kato
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Ohashi, Y., Nakai, Y., Ikeoka, H. et al. Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube. Arch Otorhinolaryngol 244, 88–90 (1987). https://doi.org/10.1007/BF00458553

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  • DOI: https://doi.org/10.1007/BF00458553

Key words

  • Ciliary activity
  • Otitis media with effusion
  • Lipopolysaccharide
  • Eustachian tube