Archives of oto-rhino-laryngology

, Volume 246, Issue 2, pp 79–82 | Cite as

Determination of lipid A in human middle ear effusions with the competitive ELISA technique

  • F. Tanimura
Original Investigations

Summary

Lipid A is a covalently bound component of gram-negative bacterial lipopolysaccharides and is the biologically active component in endotoxin. In order to detect and quantitate lipid A in middle ear effusions (MEE) from patients with otitis media with effusion (OME), antisera against lipid A were produced in mice by repeated immunization with conjugates using lipid A from Salmonella minnesota Re595 and bovine serum albumin. As a result, a competitive enzyme-linked immunosorbent assay (ELISA) was developed that was specific for lipid A. From the ELISA results, 10 out of 15 samples from the serous type MEE (mean, 13.57±2.17 μg/ml) and 5 out of 6 from the mucoid type MEE (17.73±4.34 μg/ml) were positive. These findings indicate that lipid A may contribute to the pathogenesis of both types of human OME.

Key words

Lipid A Otitis media with effusion ELISA 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • F. Tanimura
    • 1
  1. 1.Department of OtolaryngologyMunicipal Yamashiro HospitalKyotoJapan

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