Zusammenfassung
Die pathologischen Veränderungen des Corti'schen Organs wurden bei Meerschweinchen mittels der Raster- und Transmissions-Elektronenmikroskopie untersucht. Als Screening-Methode wurde die Oberfläche des experimentell geschädigten Corti'schen Organs zuerst raster-elektronenmikroskopisch untersucht.
Unser Hauptinteresse lag darin, die mögliche tonotope Anordnung einer akustischen Überstimulierung auf den entsprechenden Abschnitten des Corti'schen Organs durch eine Raster-EM-Untersuchung in toto zu dokumentieren.
Wir stimulierten längere Zeit mit Sinustönen in Schalldruckbereichen, die nicht unmittelbar Haarzellschäden erwarten ließen. Abschnitte des Corti'schen Organs, die für uns von besonderem Interesse waren, wurden in einem Spezialverfahren für die Transmissions-Elektronenmikroskopie in Epon 812 eingebettet. Dieses Verfahren erlaubt es, biologisches Material auch nach erfolgter Raster-EM-Untersuchung in seiner zellulären Feinstruktur zu beurteilen.
Summary
The pathology of the organ of Corti in guinea pigs is studied by means of SEM and TEM. It was the purpose of this study to examine the surface of an experimentally altered organ of Corti by means of SEM (screening method). As it was our main interest to find out the localization of acoustic stimulation regarding the tonotopicity on the surface of the organ of Corti, we always examined the whole cochlear by means of SEM first.
In acoustic experiments we used long-time pure-tone stimulation beyond the directly damaging sound pressure level, as well as white noise or shots for experimental damage of hair cells and supporting cells. Specimens of particular interest underwent a special procedure for a supplementary TEM examination. A prolonged and modified technique of embedding the specimens in Epon 812 permitted us to obtain supplementary data from TEM without major artifacts in the fine structure of the cells.
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Supported by Deutsche Forschungsgemeinschaft (TH 235/4) and Sonderforschungsbereich 51, Prof. R. Wetzstein, MD, Anatomische Anstalten der Universität München
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Theopold, H.M., Scheler, R. The combination of scanning and transmission electron microscopy techniques in pathology of the organ of corti in guinea pigs. Arch Otorhinolaryngol 232, 233–240 (1981). https://doi.org/10.1007/BF00457447
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DOI: https://doi.org/10.1007/BF00457447
Schlüsselwörter
- Innenohrpathologie
- Akustisches Trauma
- Raster- und Transmissions-Elektronenmikroskopie
- Morphologische Techniken
- Tierexperimente