Advertisement

Archives of Dermatological Research

, Volume 280, Issue 3, pp 171–175 | Cite as

Experimental burn-induced changes in lipid peroxide levels, and activity of superoxide dismutase and glutathione peroxidase in skin lesions, serum, and liver of mice

  • S. Kawai
  • J. Komura
  • Y. Asada
  • Y. Niwa
Original Contributions

Summary

The lipid peroxide levels and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in skin lesions, liver, and serum of mice were measured from the 15th min to 6th day after burns were inflicted on their skin. The lipid peroxide levels in the skin lesions were increased at the 24th h and on the 3rd day after the burns were inflicted. However, the SOD activity did not increase but was significantly decreased from the 30th min to 24th h. GSH-Px activity was almost undetectable in both skin and serum. The lipid peroxide levels in the serum were significantly increased and SOD activity slightly increased at the 4th h. The lipid peroxide levels in the liver were elevated at the 4th h and on the 3rd day although a slight decrease was observed at the 8th h. SOD and GSH-Px activities in the liver increased from the 4th to 24th h. The present study illustrates the changes in both burn-induced lipid peroxides in each organ and the induction mechanisms of the activities of oxygen radical scavenging enzyme, SOD, and lipid peroxide processing enzyme, GSH-Px, which responded to oxygen stress.

Key words

Lipid peroxides Superoxide dismutase Glutathione peroxidase Skin burn Liver 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Asada K, Takahashi M, Nagate M (1974) Assay and inhibitors of spinach superoxide dismutase. Agr Biol Chem 38:471–473Google Scholar
  2. 2.
    Baret A, Schiavi P, Michel P, Michelson AM, Puget K (1980) A radioimmunoassay for manganese containing superoxide dismutase. FEBS Lett 112:25–29Google Scholar
  3. 3.
    Baret A, Baeteman MA, Mattei JF, Michel P, Broussolle B, Giraud F (1981) Immunoreactive Cu SOD and Mn SOD in the circulating blood cells from normal and trisomy 21 subjects. Biochem Biophys Res Commun 98:1035–1043Google Scholar
  4. 4.
    Christophersen BO (1968) Formation of monohydroxypolyenic fatty acids from lipid peroxides by a glutathione peroxidase. Biochim Biophys Acta 164:35–46Google Scholar
  5. 5.
    Gregory EM, Fridovich I (1973) Induction of superoxide dismutase by molecular oxygen. J Bacteriol 114:543–548Google Scholar
  6. 6.
    Hodgson EK, Fridovich I (1975) The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: Inactivation of the enzyme. Biochemistry 14:5294–5299Google Scholar
  7. 7.
    Lawrence RA, Burk RF (1976) Glutathione peroxidase activitiy in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952–958Google Scholar
  8. 8.
    Little C, O'Brien PJ (1968) An intracellular GSH-peroxidase with a lipid peroxide substrate. Biochem Biophys Res Commun 31:145–150Google Scholar
  9. 9.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  10. 10.
    Maseki M, Nishigaki I, Hagihara M, Tomoda Y, Yagi K (1981) Lipid peroxide levels and lipid content of serum lipoprotein fractions of pregnant subjects with or without preeclampsia. Clin Chim Acta 115:155–161Google Scholar
  11. 11.
    Nishigaki I, Hagihara M, Hiramatsu M, Izawa Y, Yagi K (1980) Effect of thermal injury on lipid peroxide levels of rat. Biochem Med 24:185–189Google Scholar
  12. 12.
    Nishigaki I, Hagihara M, Tsunekawa H, Maseki M, Yagi K (1981) Lipid peroxide levels of serum lipoprotein fractions of diabetic patients. Biochem Med 25:373–378Google Scholar
  13. 13.
    Niwa Y, Sakane T, Miyachi Y (1984) Dissociation of the inhibitory effect of dapsone on the generation of oxygen intermediates — in comparison with that of colchicine and various scavengers. Biochem Pharmacol 33:2355–2360Google Scholar
  14. 14.
    Niwa Y, Sakane T, Miyachi Y, Ozaki M (1984) Oxygen metabolism in phagocytes of leprotic patients: enhanced endogenous superoxide dismutase activity and hydroxyl radical generation by clofazimine. J Clin Microbiol 20:837–842Google Scholar
  15. 15.
    Niwa Y, Kanoh T, Sakane T, Soh H, Kawai S, Miyachi Y (1987) The ratio of lipid peroxides to superoxide dismutase activity in the skin lesions of patients with severe skin diseases: an accurate prognostic indicator. Life Sci 40:921–927Google Scholar
  16. 16.
    Niwa Y, Kanoh T, Sakane T, Soh H, Kawai S, Miyachi Y (1987) Detection of enhanced lipid peroxide levels in patients with inflammatory skin diseases. J Clin Biochem Nutr 2:245–251Google Scholar
  17. 17.
    Niwa Y, Kasama T, Kawai S, Komura J, Sakane T, Kanoh T, Miyachi Y (1988) The effect of aging on cutaneous lipid peroxide levels and superoxide dismutase activity in guinea pigs and patients with burn. Life Sci 42:351–356Google Scholar
  18. 18.
    Oberley LW, Buettner GR (1979) Role of superoxide dismutase in cancer: A review. Cancer Res 39:1141–1149Google Scholar
  19. 19.
    Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358Google Scholar
  20. 20.
    Rabinowitch HD, Clare DA, Crapo JD, Fridovich I (1983) Positive correlation between superoxide dismutase and resistance to paraquat toxicity in the green alga chlorella sorokiniana. Arch Biochem Biophys 225:640–648Google Scholar
  21. 21.
    Sato Y, Hotta N, Sakamoto N, Matsuoka S, Ohishi N, Yagi K (1979) Lipid peroxide levels in plasma of diabetic patients. Biochem Med 21:104–107Google Scholar
  22. 22.
    Suematsu T, Kamada T, Abe H, Kikuchi S, Yagi K (1977) Serum lipoperoxide level in patients suffering from liver diseases. Clin Chim Acta 79:267–270Google Scholar
  23. 23.
    Tanaka K, Sugahara K (1980) Role of superoxide dismutase in defense against SO2 toxicity and an increase in superoxide dismutase activity with SO2 fumigation. Plant Cell Physiol 21:601–611Google Scholar
  24. 24.
    Yagi K (1976) A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 15:212–216Google Scholar
  25. 25.
    Yoshioka A, Miyachi Y, Imamura S (1987) Mechanisms of reactive oxygen species-induced skin erythema and superoxide dismutase activities in guinea pigs. Jpn J Dermatol 14:569–575Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • S. Kawai
    • 1
  • J. Komura
    • 1
  • Y. Asada
    • 1
  • Y. Niwa
    • 1
  1. 1.Department of DermatologyKansai Medical UniversityJapan

Personalised recommendations