Advertisement

Archives of Dermatological Research

, Volume 281, Issue 3, pp 178–184 | Cite as

Growth defects of melanocytes in culture from vitiligo subjects are spontaneously corrected in vivo in repigmenting subjects and can be partially corrected by the addition of fibroblast-derived growth factors in vitro

  • N. Puri
  • M. Mojamdar
  • A. Ramaiah
Original Contributions

Summary

Melanocytes cultured from uninvolved skin of untreated vitiligo subjects have decreased initial seeding capacities, manifest a lag period for the onset of the growth phase, and cannot be passaged. In contrast, melanocytes obtained from uninvolved and perilesional skin of vitiligo subjects actively repigmenting under 8-methoxy psoralen plus sunlight (PUVA) therapy have higher initial seeding capacities, grow faster without a lag period, and can be passaged to more than 12 passages. Extracts of a fetal lung fibroblast cell line (PMR-GF) that promote the growth rates and passage capacities of melanocytes from normal adult donors have been found also to promote the growth rates and passage capacities of melanocytes from the uninvolved skin of vitiligo subjects. Extracts of a fetal lung fibroblast cell line (PMR-GF), however, did not have any further stimulatory effect on the growth of melanocytes obtained from repigmenting vitiligo subjects. Melanocytes cultured from normal and untreated vitiligo subjects grew individually dispersed in the absence of PMR-GF, but tended to grow in clusters in its presence. Melanocytes from the repigmenting vitiligo subjects, however, tended to grow in clusters even in the absence of PMR-GF. These results indicate that the defective in vitro growth characteristics of melanocytes from vitiligo subjects may be related to the pathogenesis of this disease. It is possible that growth factors may be involved in the process of repigmentation in vitiligo subjects.

Key words

Melanocyte culture Vitiligo Growth factor PUVA treatment 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Aubock J, Kofler D, Sifter M, Fritsch P (1983) Application of tyrosinase assay to normal melanocytes in culture. Br J Dermatol 109:413–419Google Scholar
  2. 2.
    Eisinger M, Marko O (1982) Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin. Proc Natl Acad Sci USA 79:2018–2022Google Scholar
  3. 3.
    Eisinger M, Marko O, Ogata S-I, Old LJ (1985) Growth regulation of human melanocytes; mitogenic factors in extracts of melanoma, astrocytoma and fibroblast cell lines. Science 229:984–986Google Scholar
  4. 4.
    Gilchrest BA, Vrabel MA, Flynn E, Szabo G (1984) Selective cultivation of human melanocytes from new born and adult epidermis. J Invest Dermatol 83:370–376Google Scholar
  5. 5.
    Halaban R, Alfano FD (1984) Selective elimination of fibroblasts from cultures of normal human melanocytes. In Vitro 20:447–450Google Scholar
  6. 6.
    Halaban R, Ghosh S, Baird A (1987) bFGF is the putative natural growth factor for human melanocytes. In Vitro 23:47–52Google Scholar
  7. 7.
    Hu F, Fosnaugh RP, Lesney PF (1959) In vitro studies on vitiligo. J Invest Dermatol 33:267–280Google Scholar
  8. 8.
    Lerner AB (1971) On the etiology of vitiligo and grey hair. Am J Med 51:141–147Google Scholar
  9. 9.
    Lerner AB, Snell RS, Chanco-Turner ML, McGuire JS (1966) Vitiligo and sympathectomy. Arch Dermatol 94: 269–278Google Scholar
  10. 10.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin-phenol reagent. J Biol Chem 193:265–275Google Scholar
  11. 11.
    Moellman G, Klein-Angerer S, Scollary DA, Nordlund JJ, Lerner AB (1982) Extracellular granular material and degeneration of keratinocytes in the normally pigmented epidermis of patients with vitiligo. J Invest Dermatol 79:321–330Google Scholar
  12. 12.
    Mojamdar M, Puri N, Ramaiah A (1987) Effect of mitogenic factors extracted from fetal lung fibroblasts on the in vitro growth of melanocytes obtained from normal and vitiligo subjects. J Biosci 11:399–407Google Scholar
  13. 13.
    Naughton GK, Eisinger M, Bystryn JC (1983) Antibodies to normal human melanocytes in vitiligo. J Exp Med 158:246–251Google Scholar
  14. 14.
    Ortonne JP, Mosher DB, Fitzpatrick TB (1983) Vitiligo and other hypomelanosis of hair and skin. Plenum Medical, New YorkGoogle Scholar
  15. 15.
    Ortonne JP, Schmitt D, Thivolet J (1980) PUVA-induced pigmentation of vitiligo: scanning electron microscopy of hair follicles. J Invest Dermatol 74:40–42Google Scholar
  16. 16.
    Pathak MA, Jimbow K, Parrish JA, Kaidbey KH, Kligman AL, Fitzpatrick TB (1976) Effect of UV-A, UV-B and psoralen on in vivo human melanin pigmentation. In: Riley V (ed) Pigment cell, vol 3. Karger, Basel, pp 291–298Google Scholar
  17. 17.
    Puri N, Mojamdar M, Ramaiah A (1987) In vitro growth characteristics of melanocytes obtained from adult normal and vitiligo subjects. J Invest Dermatol 88:434–438Google Scholar
  18. 18.
    Ramaiah A (1985) Vitiligo. Ind J Dermatol Venereol Leprol 51:247–255Google Scholar
  19. 19.
    Ramaiah A, Puri N, Mojamdar M (in press) Etiology of vitiligo: a new hypothesis. Acta Derm Venereol (Stockh)Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • N. Puri
    • 1
  • M. Mojamdar
    • 1
  • A. Ramaiah
    • 1
  1. 1.Department of BiochemistryAll India Institute of Medical SciencesNew DelhiIndia

Personalised recommendations