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Cell fractionation and cytological analysis of human lymphatic cells from tonsil and blood

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Zusammenfassung

Beim Vergleich der Verteilungsprofile von Tonsillenzellen und peripheren Lymphocyten treten im diskontinuierlichen Dichtegradienten keine Unterschiede auf. Eine cytologische Analyse zeigt bei Anwendung der Kieler Nomenklatur das Auftreten von Zentrocyten, Zentroblasten, Lymphocyten und Plasmazellen in der Tonsille.

Die biologische Bedeutung dieser Resultate wird unter den Aspekten Zellproliferation und Tonsillenfunktion diskutiert.

Summary

No differences could be detected in the discontinuous density gradient when comparing the distribution pattern of tonsillar cells and peripheral lymphocytes. When the Kiel nomenclature is applied, the presence of centrocytes, centroblasts, lymphocytes and plasma cells in the tonsil is shown by a cytological analysis.

The biological importance of these results is discussed from the point of cellular proliferation and tonsillar function.

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References

  1. Akahonai, Y., Kataura, A.: The formation of immunoglobulins by human tonsillar tissue cultures. Acta haemat. jap. 36, 361–362 (1973)

    Google Scholar 

  2. Antoni, F.: A tonsilla palatina szerepe az immunrendszerben. Fül- Orr- Gégegyóg. 21, 132–137 (1975)

    Google Scholar 

  3. Antoni, F., Staub, M., Puskás, M.: A human tonsilla lymphocyták szerepe az immunvédekezésben. Orvostud. Beszam. 25, 107–118 (1974)

    Google Scholar 

  4. Ballet, J. J., Merler, E.: The separation and reactivity in vitro of a subpopulation of human lymphocytes which bind histamine. Correlation of histamin reactivity with cellular maturation. Cell. Immunol. 24, 250–269 (1976)

    Google Scholar 

  5. Boyum, A.: Separation of leucocytes from blood and bone marrow. Scand. J. clin. Lab. Invest. 21 (Suppl.), 97 (1968)

    Google Scholar 

  6. Crifó, S.: B-immunocytes of the human tonsil. Minerva otorinolaring. 24, 61–65 (1974)

    Google Scholar 

  7. Delespresse, G., Dauchleau, J., Gausset, Ph., Govaerts, A.: In vitro response of subpopulations of human tonsil lymphocytes: I. Cellular collaboration in the proliferative response to PHA an Con A. J. Immunol. 116, 437–445 (1976)

    Google Scholar 

  8. Enomoto, T., Kitashoji, N., Yagura, T., Yamamura, Y.: In vitro antibody production of human tonsil for sheep red cells (SRC). Acta oto-laryng. (Stockh.) 78, 304–308 (1974)

    Google Scholar 

  9. Falk, P.: Nachweis des Thymushormons in den Tonsillen. Arch. Ohr.-, Nas.- u. Kehlk.-Heilk. 173, 325–331 (1958)

    Google Scholar 

  10. Falk, P.: Substitutionswirkungen menschlicher Tonsillenextrakte auf das Wachstum thymektomierter Meerschweinchen. Arch. Ohr.-, Nas.- u. Kehlk.-Heilk. 175, 345–351 (1959)

    Google Scholar 

  11. Feinstem, A. R., Levitt, M.: The role of tonsils in predisposing to streptococcal infections and recurrences of rheumatic fever. New Engl. J. Med. 282, 286–291 (1970)

    Google Scholar 

  12. Fioretti, A.: Die Gaumenmandel. Stuttgart: Thieme 1961

    Google Scholar 

  13. Fontana, L., Fiorilli, M., Pissarri, S., Franchi, F., Crifo, S., Aiuti, F.: Sintesi in vitro delle IgE nei tessuti umani. Folia allerg. (Roma) 20, 163–168 (1973)

    Google Scholar 

  14. Geha, R. S., Merler, E.: Response of human thymus-derived (T) and non-thymus-derived (B) lymphocytes to mitogenic stimulation in vitro. Europ. J. Immunol. 4, 193–199 (1974)

    Google Scholar 

  15. Geha, R. S., Schneeberger, E., Rosen, F. S., Merler, E.: Interaction of human thymus-derived and non-thymus-derived lymphocytes in vitro. J. exp. Med. 138, 1230–1247 (1973)

    Google Scholar 

  16. Grobachevsky, V. N., Grinevich, Yu. A.: Concentration of immunoglobulins in stomato pharyngeal secretion in healthy people, patients with chronic tonsillitis and people with removed palatine tonsils (russ.). Zh. ushn. nos. gorlov. Bolezn. 36, 10–14 (1976)

    Google Scholar 

  17. Harrison, B. H.: Tonsillar tissue implants in neonatally thymectomized mice. J. Immunol. 105, 38–42 (1970)

    Google Scholar 

  18. Harrison, B. H.: Antibody production in thymectomized mice with implants of rabbit tonsillar tissue. Transplantation 14, 402–403 (1972)

    Google Scholar 

  19. Hoffmann, M. K., Schmidt, D., Oettgen, H. F.: Production of antibody to sheep red blood cells by human tonsil cells in vitro. Nature (Lond.) 243, 408–410 (1973)

    Google Scholar 

  20. Hurtado, R. C., Rola-Pleszczynski, M., Merida, M. A., Hensen, S. A., Vincent, M. M., Thong, Y. H., Bellanti, J. A.: The immunologic role of tonsillar tissues in local cell-mediated immune responses. J. Pediat. 86, 405–408 (1975)

    Google Scholar 

  21. Ishikawa, T., Shimada, T., Fujita, Y., Ishikawa, M.: Antibody production in human tonsil: detection of IgE antibody forming tonsil lymphocytes. Auris. Nasus. Larynx 2, 91–96 (1975)

    Google Scholar 

  22. Ishikawa, T., Shimada, T., Miyashita, H., Ishikawa, M.: Antibody production in human tonsil: detection of antibody forming cell and antigen binding cell to Dermatophagoides farinae. Auris. Nasus. Larynx 2, 83–90 (1975)

    Google Scholar 

  23. Ishikawa, T., Wicher, K., Arbesman, C. E.: Distribution of immunoglobulins in palatine and pharyngeal tonsils. Int. Arch. Allergy 43, 801–812 (1972)

    Google Scholar 

  24. Kataura, A., Akahonai, Y.: Immunoglobulin synthesis of the human tonsil in vitro. Auris. Nasus. Larynx. 3, 127–132 (1976)

    Google Scholar 

  25. Klimpel, G. R., Day, K. D., Lucas, D. O.: Differential production of interferon and lymphotoxin by human tonsil lymphocytes. Fed. Proc. 34, 978 (1975)

    Google Scholar 

  26. Koburg, E.: Die Tonsille im immunologischen Geschehen. Arch. klin. exp. Ohr.-, Nas.- u. Kehlk.-Heilk. 196, 65–75 (1970)

    Google Scholar 

  27. Lai A Fat, R. F. M., van Furth, R.: In vitro synthesis of some complement components (Clq, C3 and C4) by lymphoid tissues and circulating leucocytes in man. Immunology 28, 359–367 (1975)

    Google Scholar 

  28. Lennert, K., Mohri, N., Stein, H., Kaiserling, E.: The histopathology of malignant lymphoma. Brit. J. Haemat. 31, 193 (1975)

    Google Scholar 

  29. Lennert, K., Müller-Hermelink, H.-K.: Lymphozyten und Funktionsformen — Morphologie, Organisation und immunologische Bedeutung. Verh. anat. Ges. (Jena) 69, 19–62 (1975)

    Google Scholar 

  30. Litwin, D. S., Huettenroth, T. H., Balaban, L.: Synthesis of Gm allotypes by human tonsillar lymphocytes in culture: concordance with serum phenotype. J. Immunol. 116, 1045–1050 (1976)

    Google Scholar 

  31. Lundak, R. L., Galant, S. P.: In vitro antigen specific suppression of a subpopulation of IgM synthesizing cells in human tonsils. Fed. Proc. 34, 1004 (1975)

    Google Scholar 

  32. Merétey, K., Köteles, G. J., Elekes, E.: Natural antibody production in human tonsils. Experientia (Basel) 28, 457–458 (1972)

    Google Scholar 

  33. Merler, E., Silberschmidt, M.: Uptake of antigen by human lymphocytes. Immunology 22, 821–831 (1972)

    Google Scholar 

  34. Meyer zum Gottesberge, A., Koburg, E.: Die Tonsille als lymphozytäres Organ. Acta oto-laryng. (Stockh.) 63, 229–237 (1967)

    Google Scholar 

  35. Mitrou, P. S., Queisser, M., Lennert, K., Sandritter, M.: Kombinierte autoradiographisch-zytophotometrische Untersuchungen von Keimzentrumszellen der menschlichen Tonsille. Virchows Arch. Abt. B Zellpath. 3, 156–170 (1969)

    Google Scholar 

  36. Morag, A., Morag, B., Bernstein, J. M.: In vitro correlates of cell mediated immunity in human tonsils after natural or induced rubella virus infection. J. infect. Dis. 131, 409–416 (1975)

    Google Scholar 

  37. Müller-Hermelink, H. K., Caesar, R.: Elektronenmikroskopische Untersuchungen der Keimzentren in menschlichen Tonsillen. Z. Zellforsch. 96, 521–547 (1969)

    Google Scholar 

  38. Newmann, W., Stoner, G. L., Bloom, B. R.: Primary in vitro sensitisation of human T-cells. Nature (Lond.) 269, 151–153 (1977)

    Google Scholar 

  39. Ogra, P. L., Morag, A., Beutner, K. R.: Amplification of local immune responses with active immunisation. 4th Int. Convoc. Immunol. 1974, pp. 323–333. Basel: Karger 1975

    Google Scholar 

  40. Ostergaad, P. A.: Presence of immunoglobulin-containing plasma cells in tonsils from tonsillectomiced children. Acta path. microbiol. scand. 83C, 406–412 (1975)

    Google Scholar 

  41. Piffkó, P., Köteles, G. J., Antoni, F.: Biochemical properties of human tonsillar lymphocytes. Pract. oto-rhino-laryng. (Basel) 32, 350–355 (1970)

    Google Scholar 

  42. Platts-Mills, T. A. E., Ishizaka, K.: IgG and IgA diphtheria antitoxin responses from human tonsil lymphocytes. J. Immunol. 114, 1058–1064 (1975)

    Google Scholar 

  43. Platts-Mills, T. A. E., Ishizaka, K.: IgG diphtheria antitoxin responses from human tonsil lymphocytes induced by anti-γ-chain antibodies. J. Immunol. 114, 1605–1010 (1975)

    Google Scholar 

  44. Puskás, M., Antoni, F., Staub, M., Farkas, Gy., Piffkó, P.: Lysocyme activity of the human tonsil. Pract. oto-rhino-laryng. (Basel) 34, 160–164 (1972)

    Google Scholar 

  45. Puskás, M., Staub, M., Antoni, F.: In vitro synthesis and identification of lysocyme in human tonsillar cells. Biochem. Med. 13, 359–369 (1975)

    Google Scholar 

  46. Siegel, G.: Proliferation of cells and intracellular c-AMP-content: comparative investigations of autolog lymphatic cells from palatine tonsils, nasopharyngeal tonsils and blood. Arch. ORL 220, 41–46 (1978)

    Google Scholar 

  47. Siegel, G., Wilke, J.: Concentration of immunoglobulin in the tonsils. ORL 38, 246–251 (1976)

    Google Scholar 

  48. Siegel, G., Wilke, J.: The accumulation of immunoglobulins in the tonsil. Acta biol. med. germ. 35, 1031–1033 (1976)

    Google Scholar 

  49. Sloyer, J. L., Veltri, L. W., Sprinkle, P. M.: In vitro IgM antibody synthesis by human tonsilderived lymphocytes. J. Immunol. 111, 183–188 (1973)

    Google Scholar 

  50. Smith, R. S., Sherman, N. A., Newcomb, R. W.: Synthesis and secretion of immunoglobulins, including IgA, by human tonsil and adenoid tissue cultured in vitro. Int. Arch. Allergy 46, 785–801 (1974)

    Google Scholar 

  51. Sordat, B., Hess, M. W., Cottier, H.: IgG immunoglobulin in the wall of post-capillary venules: possible relationship to lymphocyte recirculation. Immunology 20, 115–118 (1971)

    Google Scholar 

  52. Staub, M.: DNA-Polymerase activity in human tonsillar lymphocytes. 9th FEBS-Meeting Abstr., p. 163 s4a2, Budapest, 25.–30. 8. 1974

  53. Sugiyama, M., Yamamoto, K., Kinoshita, Y., Kimura, S.: Studies on differences in cellular immune-response between the human tonsil and blood lymphocytes. Acta oto-laryng. (Stockh.) 82, 440–450 (1976)

    Google Scholar 

  54. Sugiyama, M., Yamamoto, K., Kinoshita, Y., Kimura, S.: Studies on the capacity of human tonsillar lymphocyte subpopulations to produce interferon. Acta oto-laryng. (Stockh.) 84, 296–305 (1977)

    Google Scholar 

  55. Surján, L., Sen., Surján, M.: Immunological role of human tonsils. Acta oto-laryng. (Stockh.) 71, 190–193 (1971)

    Google Scholar 

  56. Surján, L., Sen., Surján, L., Jun., Surján, M.: Further investigations into the immunological role of tonsils. Acta oto-laryng. (Stockh.) 73, 222–226 (1972)

    Google Scholar 

  57. Surján, L.: Immunological significance of the lymphoidepithelial organs (tonsils). XI. Congresso Mundal de Otorhinolaryngologica, Abstract, p. 2–3, Buenos Aires 13.–19. 3. 1977

  58. Suzuki, M., Ino, H.: Relation between thymus and tonsil. Arch. klin. exp. Ohr.-, Nas.- u. Kehlk.-Heilk. 204, 249–260 (1973)

    Google Scholar 

  59. Szabó, M. T., Antoni, F.: A “Cytotoxic” factor released from unactivated human lymphocytes. Acta biochim. Biophys. Acad. Sci. hung. 10, 31–36 (1975)

    Google Scholar 

  60. Szabó, M. T., Hrabák, A., Antoni, F.: Radiochromate-binding capacity of human tonsillar lymphocytes. J. nucl. Med. 15, 750–755 (1975)

    Google Scholar 

  61. Tabata, T., Enomoto, T., Fujimura, N., Hiramatsu, K.: Immunological function of human tonsils, with special reference to E- and EAC-binding lymphocytes. Acta oto-laryng. (Stockh.) 77, 150–154 (1974)

    Google Scholar 

  62. Tabata, T., Katsurahara, T., Enomoto, T., Kitashoji, N., Tanaka, S.: Immunological function of human tonsil. Acta oto-laryng. (Stockh.) 80, 474–478 (1975)

    Google Scholar 

  63. Tsigamov, A., Vizirenko, I.: Immunologic competence of lymphoid tissue of palatine tonsils. XI. Congresso Mundal de Otorhinolaryngologica, Abstract, p. 318, Buenos Aires 13.–19. 3. 1977

  64. Veltri, R. W., McClung, J. E., Sprinkle, P. M.: Epstein Barr nuclear antigen (EBNA) carrying lymphocytes in human palatine tonsils. J. gen. Virol. 32, 455–460 (1976)

    Google Scholar 

  65. Vershigora, A. E., Vizirenko, L. V., Grinevich, Yu. A.: The palatal tonsils and immunity (russ.). Zh. Mikrobiol. (Mosk.) 50, 31–33 (1973)

    Google Scholar 

  66. Vizirenko, L. V., Kondratsova, T. P., Vershigora, A. E.: Palatine tonsils and immunity VII. Soluble factors produced in vitro by lymphocytes of the palatine tonsils (russ.). Zh. Mikrobiol. (Mosk.) 52, 82–85 (1975)

    Google Scholar 

  67. Waksman, B. H., Ozer, H.: Specialized amplification elements in the immune system. Progr. Allergy 21, 1–113 (1976)

    Google Scholar 

  68. Watanabe, T., Yoshizaki, K., Yagura, T., Yamamura, Y.: In vitro antibody formation by human tonsil lymphocytes. J. Immunol. 113, 608–616 (1974)

    Google Scholar 

  69. Willson, J. K. V., Zaremba, J. L., Pitts, A. M., Pretlow, T. G.: A characterization of human tonsillar lymphocytes after separation from other tonsillar cells in an isokinetic gradient of Ficoll in tissue culture medium. Amer. J. Path. 83, 341–358 (1976)

    Google Scholar 

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Siegel, G. Cell fractionation and cytological analysis of human lymphatic cells from tonsil and blood. Arch Otorhinolaryngol 221, 15–21 (1978). https://doi.org/10.1007/BF00456380

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