Summary
The effect of porcine gastric inhibitory polypeptide on hepatic glycogen metabolism was investigated in the isolated in situ perfused rat liver. Glycogenolysis was stimulated by infusion of glucagon into the portal vein (half maximal effective portal vein concentration ∼30 pmol/1). When glucagon was infused at a final portal vein concentration of 0.5 nmol/l, simultaneous addition of insulin inhibited the glucagon-dependent glycogenolysis in a dose-dependent way (half maximal effective concentration for insulin about 2 nmol/l). Gastric inhibitory polypeptide alone at a concentration of 1 nmol/l reduced glucagon-dependent glycogenolysis only slightly. However, when infused simultaneously at low insulin concentrations (0.1 nmol/1), gastric inhibitory polypeptide suppressed hepatic glucose production dose-dependently up to 70%. The data suggest that gastric inhibitory polypeptide exerts direct metabolic effects on hepatic glycogen metabolism predominantly in a situation where insulin is simultaneously present, e.g. following ingestion of glucose.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Brown JC, Dryburgh JR, Ross SA, Dupré J (1975) Identification and actions of gastric inhibitory polypeptide. Rec Progr Horm Res 31: 487–532
Pederson RA, Brown JC (1976) The insulinotropic action of gastric inhibitory polypeptide in the perfused isolated rat pancreas. Endocrinology 99: 780–785
Creutzfeldt W (1979) The incretin concept today. Diabetologia 16: 75–85
Dupré J, Greenridge N, McDonald TJ, Ross SA, Rubenstein D (1976) Inhibition of actions of glucagon in adipocytes by gastric inhibitory polypeptide. Metabolism 25: 1197–1199
Ebert R, Brown JC (1976) Effect of gastric inhibitory polypeptide (GIP) on lipolysis and cyclic AMP levels in isolated fat cells. Eur J Clin Invest 6: 327 (Abstract)
Eckel RH, Fujimoto WY, Brunzell JD (1979) Gastric inhibitory polypeptide enhanced lipoprotein lipase activity in cultured preadipocytes. Diabetes 28: 1141–1142
Wasada T, McCorkle K, Harris V, Kawai K, Howard B, Unger RH (1981) Effect of gastric inhibitory polypeptide on plasma levels of chylomicron triglycerides in dogs. J Clin Invest 68: 1106–1107
Hanks JB, Meyers WC, Andersen DK, Hill RC, Jones RS (1979) The effects of gastric inhibitory polypeptide (GIP) on hepatic glucose production. Gastroenterology 76: 1150 (Abstract)
Ebert R, Arends J, Creutzfeldt W (1983) Insulin-dependent suppression of hepatic glucose output by GIP in the perfused rat liver. Regul Pept 1 [Suppl 2]: 162 (Abstract)
Andersen DK, Sun YS, Brunicardi FC, Berlin SA, Lebovitz HE, Garksy V, (1984) Regulation of hepatic glucose production glucagon (GLUC). Dig Dis Sci 29 [August Suppl]: 6S (Abstract)
Jungermann K, Heilbronn R, Katz N, Sasse D (1982) The glucose/glucose-6-phosphate cycle in the periportal and perivenous zone of rat liver. Eur J Biochem 123: 429–436
Hartmann H, Beckh K, Jungermann K (1982) Direct control of glycogen metabolism in the perfused rat liver by the sympathetic innervation. Eur J Biochem 123: 521–526
Dhumeaux D, Berthelot P (1973) Measurement of hepatic blood flow in the rat. Transhepatic catheterization of the hepatic veins. Biol Gastroenterol 6: 49–54
Ossenburg EW, Danis P, Benhamou JP (1974) Hepatic blood flow in the rat: effect of portocaval shunt. J Appl Physiol 37: 806–808
Bergmeyer HU (ed) (1974) Methoden der Enzymatischen Analysen, 3. Aufl, Bd 2. Verlag Chemie, Weinheim
Hue L, Van de Werve G (1981) (eds) Short-term regulation of liver metabolism. Elsevier/North Holland Biomedical Press, Amsterdam New York
Hems DA, Whitton PD (1980) Control of hepatic glycogenolysis. Physiol Rev 60:1–50
Lautt WW (1983) Afferent and efferent neural roles in liver function. Progr Neurobiol 21: 323–348
Mackrell DJ, Sokal JE (1969) Antagonism between the effects of insulin and glucagon in the isolated liver. Diabetes 18: 724–732
Hanks JB, Andersen DK, Wise JE, Putnam WS, Meyers WC, Jones RS (1984) The hepatic extraction of gastric inhibitory polypeptide and insulin. Endocrinology 115:1011–1018
Beckh K, Hartmann H, Jungermann K (1982) Modulation by insulin and glucagon of the activation of glycogenolysis by perivascular nerve stimulation in the perfused rat liver. FEBS Lett 146: 69–72
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hartmann, H., Ebert, R. & Creutzfeldt, W. Insulin-dependent inhibition of hepatic glycogenolysis by gastric inhibitory polypeptide (GIP) in perfused rat liver. Diabetologia 29, 112–114 (1986). https://doi.org/10.1007/BF00456120
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00456120