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Archives of Microbiology

, Volume 152, Issue 2, pp 201–205 | Cite as

One-carbon compounds transport studies in the obligate methylotroph

  • N. Borisov
  • A. Netrusov
Original Papers

Abstract

Cells of obligate methylotrophic Gram-negative bacterium Methylobacillus flagellatum KT which can only grow on methanol and methylamine media possess three different carriers mediating uptake of methylamin depending on growth conditions. All three uptake systems are energy-dependent, the methylamine uptake was inhibited by oxidative phosphorylation uncoupler and respiratory inhibitors. The first active transport system for methanol in the cells of obligate methylotroph was also demonstrated. The parameters of this system were measured, their dependence on energy, presence of respiratory inhibitors and uncoupler was shown.

Key words

Methylobacillus flagellatum KT Methylammonium transport Methanol transport Nitrogen control Carbon control 

Abbreviations

CCCP

Carbonyl cyanide p-(trichloromethoxy)-phenylhydrazone

DCCD

N,N′-dicyclohexyl-carbodiimide

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • N. Borisov
    • 1
  • A. Netrusov
    • 1
  1. 1.Department of Microbiology, Faculty of BiologyMoscow State UniversityMoscowUSSR

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