Archives of Microbiology

, Volume 101, Issue 1, pp 337–342 | Cite as

Formation of protoplasts in Azotobacter vinelandii

  • Kai Yip Cho
  • Leodocia Pope
  • Orville Wyss


Protoplasts of Azotobacter vinelandii were formed by incubating whole cells in lysozyme and EDTA in Tris-HCl buffer (0.05 M, pH 8.0) supplemented with sucrose (15% w/v). This appeared to be related to the special chelating ability of EDTA and Tris-HCl since substitution of the former by nitrilotriacetic acid or by trisodium citrate and the latter by veronal-acetate buffer or tris-maleate buffer over a pH range of 5.2 to 8.6 yielded only spheroplasts. Of nine strains of Azotobacter studied, only A. vinelandii strain 12837 and strain 0 formed protoplasts.

Key words

Protoplasts Azotobacter Cell Wall Plasma Membrane 


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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Kai Yip Cho
    • 1
  • Leodocia Pope
    • 1
  • Orville Wyss
    • 1
  1. 1.Department of MicrobiologyUniversity of Texas at AustinAustin

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