Summary
The respiratory quotient of the stria vascularis was measured in vitro by means of Cartesian diver microgasometry. A value of about 1.2 was found when the incubation medium was phosphate-buffered serum substitute with glucose as the sole substrate. This value suggests that endogenous lipids and amino acids do not contribute significantly to strial respiratory metabolism and that carbohydrate is the primary fuel in vitro. A high activity of the hexose monophosphate pathway may be responsible for raising the respiratory quotient above unity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ashmore, J., Konishita, J. H., Nesbett, F. B., Hastings, A. B.: Studies on carbohydrate metabolism in rat liver slices. J. biol. Chem. 220, 619–626 (1956)
Bartley, W., Birt, L. M., Banks, P.: The Biochemistry of the Tissues. New York: John Wiley and Sons 1970
Bass, A., Brdiczba, D., Eyer, P., Hofer, S., Pette, D.: Differentiation of distinct muscle types at the level of enzymatic organization. Europ. J. Biochem. 10, 198–206 (1969)
Borrow, A., Penny, J. R.: Further observations on biological reactions by the Cartesian diver technique — with special reference to mammalian tissues. Exp. Cell Res. 2, 188–206 (1951)
Chou, J. T. Y., Rodgers, K.: Respiration of tissue lining the mammalian membranous labyrinth. J. Laryng. 76, 341–351 (1962)
Davis, H.: A model for transducer action in the cochlea. Cold Spr. Harb. Symp. quant. Biol. 30, 181–190 (1965)
Dickens, F., Simer, F.: The metabolism of normal and tumour tissue. IV. The respiratory quotient in bicarbonate media. Biochem. J. 25, 985–993 (1931)
Echandia, E. L. R., Burgos, M. H.: The fine structure of the stria vascularis of the guinea pig inner ear. Z. Zellforsch. 67, 600–619 (1965)
Elliott, K. A. C., Greig, M. E., Benoy, M. P.: The metabolism of lactic and pyruvic acids in normal and tumour tissues. III. Rat liver, brain, and testis. Biochem. J. 31, 1003–1020 (1937)
Fitch, W. M., Chaikoff, I. L.: Extent and patterns of adaptation of enzyme activities in livers of normal rats fed diets high in glucose and fructose. J. biol. Chem. 235, 554–557 (1960)
Glock, G. E., McLean, P.: A preliminary investigation of the hormonal control of the hexose monophosphate oxidative pathway. Biochem. J. 61, 390–397 (1955a)
Glock, G. E., McLean, P.: Effects of hormones on levels of oxidized and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotides in liver and diaphragm. Biochem. J. 61, 397–402 (1955b)
Holter, H.: Technique of the Cartesian diver. C.R. Lab. Carlsberg (Ser. Chim.) 24, 400–478 (1943)
Lehninger, A. L.: Biochemistry. P. 338. New York: Worth Publishers 1970
Linderstrøm-Lang, K.: On the theory of the Cartesian diver microrespirometer. C.R. Lab. Carlsberg (Ser. Chim.) 24, 333–398 (1943)
Marcus, D. C., Thalmann, R., Marcus, N. Y.: Respiratory rate and ATP conten of stria vascularis of guinea pig in vitro. Laryngoscope (1978) (in press)
Matschinsky, F. M., Thalmann, R.: Quantitative histochemistry of the organ of Corti, stria vascularis and macula sacculi of the guinea pig. I. Sampling procedure and analysis of pyridine nucleotides. Laryngoscope (St. Louis) 77, 292–305 (1967)
McIlwain, H., Bachelard, H. S.: Biochemistry and the Central Nervous System. London: Churchill Livingstone 1971
Tachibana, M., Mizukoshi, O., Kuriyama, K.: Inhibitory effects of kanamycin on glycolysis in cochlea and kidney — possible involvement in the formation of oto- and nephrotoxicities. Biochem. Pharmacol. 25, 2297–2301 (1976)
Thalmann, R.: Metabolic features of auditory and vestibular systems. Laryngoscope (St. Louis) 81, 1245–1260 (1971)
Thalmann, R., Miyoshi, T., Thalmann, I.: The influence of ischemia upon energy reserves of inner ear tissues. Laryngoscope (St. Louis) 82, 2249–2272 (1972)
Waterlow, J. C., Borrow, A.: Experimental observations on the Cartesian diver technique — with special reference to mammalian tissue. C.R. Lab. Carlsberg (Ser. Chim.) 27, 93–123 (1949)
Weidemann, M. J., Krebs, H. A.: The fuel of respiration of rat kidney cortex. Biochem. J. 112, 149–166 (1969)
Wood, H. G., Katz, J., Landau, B. R.: Estimation of pathways of carbohydrate metabolism. Biochem. Z. 338, 809–847 (1963)
Author information
Authors and Affiliations
Additional information
Supported by the grant NS 06575 from the National Institutes of Health and the grant 77-16842 from the National Science Foundation
Rights and permissions
About this article
Cite this article
Marcus, D.C., Thalmann, R. & Marcus, N.Y. Respiratory quotient of stria vascularis of guinea pig in vitro. Arch Otorhinolaryngol 221, 97–103 (1978). https://doi.org/10.1007/BF00455880
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00455880