Advertisement

Mycopathologia

, Volume 101, Issue 1, pp 25–29 | Cite as

Production of sterigmatocystin by Aspergillus versicolor isolated from roughage

  • Peter Lepom
  • Helga Kloss
Article

Abstract

A total of 69 samples of hay and straw collected during the winter period of 1984/85 were surveyed for their contamination by Aspergillus versicolor. The percentage of A. versicolor-positive samples was 14.5%. Nineteen A. versicolor strains mainly isolated from roughage were tested for the production of sterigmatocystin. All of the isolates examined were capable of producing different levels of sterigmatocystin on a cracked corn substrate. The majority of these strains were highly toxigenic; 53% of the isolates produced more than 500 mg/kg of sterigmatocystin. These findings suggest that corn is a very suitable substrate for sterigmatocystin production and that particularly in the surface layers of feed stocks and corn silos such toxigenic strains of A. versicolor can produce considerable growth and possibly sterigmatocystin, too.

Key words

Aspergillus versicolor sterigmatocystin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Abramson D, Mills JT, Boycott BR. Mycotoxins and mycoflora in animal feedstuffs in western Canada. Can J Comp Med 1983 47:23–26.Google Scholar
  2. 2.
    Buckle AE. The occurrence of mycotoxins in cereals and animal feedstuffs. Vet Res Commun 1983 7:171–186.Google Scholar
  3. 3.
    Christensen CM, Kaufmann HH. Mycoflora. In: Christensen CM, ed. Storage of cereal grains and their products. St. Paul. MM: American Association of Cereal Chemists, 1974:158–192.Google Scholar
  4. 4.
    Devi RG, Polasa H. Mycotoxins from fungi on maize. Curr Sci 1982; 51:751.Google Scholar
  5. 5.
    Juskiewicz T, Piskorska-Pilszczynska J. Content of mycotoxins in industrial mixed feeds and concentrates. Med Weter 1977; 33:193–196.Google Scholar
  6. 6.
    Le Bars J. Bioproduction de sterigmatocystine par l'Aspergillus versicollor (Vuill.) Tiraboschi dans les fourrages secs. Ann Nutr Alim 1977; 31:575–581.Google Scholar
  7. 7.
    Le Bars J. Production of sterigmatocystin by Aspergillus versicolor (Vuill.) Tir.: Frequency of toxigenic strains isolated from dry fodders. Zesz Probl Postepow Nauk Roln 1977; 189:105–111.Google Scholar
  8. 8.
    Mislivec PB, Bruce VR. Direct plating versus dilution plating in qualitatively determining the mold flora of dried beans and soybeans. J Assoc Off Anal Chem 1977; 60:741–743.Google Scholar
  9. 9.
    Official Methods of Analysis of the AOAC, 14th edition. Arlington, VA: AOAC, 1984: sections 26.132–26.138.Google Scholar
  10. 10.
    Orth R. Occurrence and estimation of sterigmatocystin-producing strains of Aspergillus versicolor in foodstuffs. Zesz Probl Postepow Nauk Roln 1977; 189:25–33.Google Scholar
  11. 11.
    Orth R. Einfluss physikalischer Faktoren auf die Bildung von Mykotoxinen. In: J Reiss, ed. Mykotoxine in Lebensmitteln. Stuttgart/New York: Gustav Fischer Verlag, 1981:85–100.Google Scholar
  12. 12.
    Rabie CJ, Lübben A, Steyn M. Production of sterigmatocystin by Aspergillus versicolor and Bipolaris sorokiniana on semisynthetic liquid and solid media. Appl Environ Microbiol 1976; 32:206–208.Google Scholar
  13. 13.
    Raper KB, Fennell DI. The genus Aspergillus. Baltimore: Williams and Wilkins, 1965.Google Scholar
  14. 14.
    Sandor G. Occurrence of mycotoxins in feeds, animal organs and secretions. Acta Vet Hungarica 1984; 32:57–69.Google Scholar
  15. 15.
    Schroeder HW, Kelton WH. Production of sterigmatocystin by some species of the genus Aspergillus and its toxicity to chicken embryos. Appl Microbiol 1975; 30:589–591.Google Scholar
  16. 16.
    Scott PM, Van Walbeek W, Kennedy B, Anyeti D. Mycotoxins (ochratoxin A, citrinin, and sterigmatocystin) and toxigenic fungi in grains and other agricultural products. J Agric Food Chem 1972; 20:1103–1109.Google Scholar
  17. 17.
    Shreeve BG, Patterson DSP, Roberts BA. Investigations of suspected cases of mycotoxicosis in farm animals in Britain. Vet Rec 1975; 97:275–278.Google Scholar
  18. 18.
    Steyn M, Rabie CJ. Production of sterigmatocystin. J Assoc Off Anal Chem 1975; 58:622–623.Google Scholar
  19. 19.
    Thalmann A, Möller IM. Methodische Versuche zur Keimzahlbestimmung bei Futtermitteln. Die Bodenkultur 1973; 24:402–406.Google Scholar
  20. 20.
    Vesonder RF, Horn BW. Sterigmatocystin in dairy cattle feed contaminated with Aspergillus versicolor. Appl Environ Microbiol 1985; 49:234–235.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1988

Authors and Affiliations

  • Peter Lepom
    • 1
  • Helga Kloss
    • 1
  1. 1.Academy of Agricultural Sciences of the German Democratic RepublicInstitute of Forage Production PaulinenauePaulinenaueGDR

Personalised recommendations