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Ultrastructural localization of acid phosphatase in human laryngeal carcinoma

Ultrastrukturelle Lokalisation von saurer Phosphatase beim Kehlkopfkarzinom des Menschen

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Zusammenfassung

Die subzelluläre Verteilung von saurer Phosphatase in malignen Keratinozyten des invasiven Larynxkarzinoms wurde mit Hilfe der ultrastrukturellen Zytochemie untersucht. Das Reaktionsprodukt war im Golgi-Apparat, endoplasmatischen Retikulum und in einigen zytoplasmatischen Vesikeln lokalisiert. Saure Phosphatase-Aktivität wurde auch in lysosomalen Strukturen im kortikalen Zytoplasma der basalen Karzinomzellen beobachtet. Extrazellulär fand sich Phosphatase-Aktivität in membranbegrenzten Strukturen an der Tumor-Stromagrenze. Die Lokalisation der sauren Phosphatase in der vorliegenden Arbeit wird in Hinblick auf saure Phosphatase-Aktivität in anderen Tumoren erörtert. Die Ergebnisse unterstreichen die bedeutende Rolle, welche der Freisetzung von hydrolytischen Enzymen bei der Tumorinvasion in umgebende Gewebe zukommt.

Summary

The subcellular distribution of acid phosphatase in malignant keratinocytes of invasive laryngeal carcinoma was studied by ultrastructural cytochemistry. The reaction product was localized in Golgi, ER, and some cytoplasmic vesicles. Acid phosphatase activity was also observed in lysosomal structures in the cortical cytoplasm of basal carcinoma cells. Extracellular acid phosphatase activity also occurred at the tumor-stroma junction in membrane-bound vesicular structures. The localization of acid phosphatase in this report is discussed in relation to acid phosphatase activity in other tumors. The findings lend further support to the important role of hydrolytic enzyme release in respect to tumor invasion into surrounding tissues.

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Authors and Affiliations

  1. Department II of Otolaryngology, University of Vienna, Alserstraße 4, A-1090, Vienna, Austria

    P. Schenk

  2. Department I of Dermatology, University of Vienna, Alserstraße 4, A-1090, Vienna, Austria

    K. Konrad

Authors
  1. P. Schenk
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  2. K. Konrad
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Additional information

This work was supported by the “Krebsausschuß der Medizinischen Fakultät der Universität Wien” and by the “Fonds Österreichischer Krebsforschungsinstitute”

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Schenk, P., Konrad, K. Ultrastructural localization of acid phosphatase in human laryngeal carcinoma. Arch Otorhinolaryngol 226, 213–218 (1980). https://doi.org/10.1007/BF00455582

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  • DOI: https://doi.org/10.1007/BF00455582

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