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T-Cell function in atopic dermatitis controlled examination of Concanavalin A dose-response relations in cultured lymphocytes

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Summary

The Concanavalin A (Con A) stimulation response of peripheral lymphocytes in vitro was found to be decreased statistically significant (α=0.005) in patients with light atopic dermatitis, Strictly matched pairs were examined. Using ten graded concentrations of Con A, dose-response relations of the pairs were demonstrated, differences were more evident at lower suboptimal concentrations. Background DNA-synthesis of the patients was elevated.

Zusammenfassung

Bei Patienten mit Neurodermitis atopica leichten Schweregrades konnte im Rahmen einer kontrollierten Studie mit Hilfe von “matched pairs” eine gegenüber gesunden Kontrollpersonen signifikant (α=0,005) herabgesetzte in vitro 3H-Thymidin-Einbaurate in kultivierte Lymphozyten des peripheren Blutes, nach Stimulation mit dem T-Zellenmitogen Concanavalin A (Con A), festgestellt werden. Anhand von zehn differenten Konzentrationen von Con A wurden die Dosis-Wirkungsbeziehungen innerhalb der Paare bestimmt. Während höhere Con A-Konzentrationen in den Lymphozytenkulturen zu optimalen 3H-Thymidin-Einbauraten in neusynthetisierte DNS führten, waren die Unterschiede zwischen Neurodermitikern und Kontrollpersonen eindeutiger bei niedrigeren, suboptimalen Con A-Konzentrationen. Die spontane 3H-Thymidin-Einbaurate in kultivierte Lymphozyten ohne Con A-Stimulation (spontane DNS-Synthese) war bei Neurodermitikern im Vergleich zu Kontrollpersonen erhöht. Die Befunde weisen auf eine beeinträchtigte T-Zellenfunktion bei Neurodermitikern hin.

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Authors and Affiliations

  1. Abt. Dermatologie II, Schwerpunkt: Allergien und Berufskrankheiten der Haut, Klinikum der Universität Heidelberg, Hautklinik (Geschäftsführender Ärztlicher Direktor: Prof. Dr. U. W. Schnyder), Voßstraße 2, 6900, Heidelberg 1, Federal Republic of Germany

    E. Schöpf (Ärztlicher Direktor)

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  1. E. Schöpf
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  2. A. Kapp
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  3. C. W. Kim
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Substantial parts of this study formed the object of A. Kapp's doctoral thesis, Medizinische Fakultät der Universität Heidelberg

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Schöpf, E., Kapp, A. & Kim, C.W. T-Cell function in atopic dermatitis controlled examination of Concanavalin A dose-response relations in cultured lymphocytes. Arch Dermatol Res 262, 37–44 (1978). https://doi.org/10.1007/BF00455571

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  • DOI: https://doi.org/10.1007/BF00455571

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