Summary
A simplified method is described to demonstrate Y chromosome fluorescence in interphase nuclei of epithelial cells in human buccal smears using Quinacrine Mustard. This technique turned out to be efficient in our hands and would be of value in determining the sex chromosomes in the epithelial cells of the skin in cases of allogeneic transplantations from females to males and vice versa to determine the period during which the grafts survived.
Zusammenfassung
Es wurde eine vereinfachte Methode zur Darstellung der Y-Chromosomem-Fluorescenz in epithelialen Zellen der Haut, die sich in der Interphase befinden, entsprechend den Wangenschleimhautabstrichen zur Darstellung von Sexchromosomen beschrieben. Zur Fluorescenzdarstellung wurde “Anhydrous Quinacrine Mustard Dihydrochloride (Sigma Chem. Co. U.S.A.)” verwandt. Die Technik bewährte sich und ist geeignet, die Sexchromosomen in den Epithelzellen der Haut in Fällen allogenetischer Transplantation von weiblichen auf männlichen und vice versa Probanden in der Zeit zu bestimmen, wo die Transplantationen überlebten.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baccichetti, C., Tenconi, R., Largaiolli, R.: Screening test for Y chromosome in new born using urinary epithelial cells. Lancet 2, 1328 (1971)
Barr, M. L., Bertram, E. G.: A morphological distinction between neurons of the male and female and the behaviour of the nucleolar satellite during accelerated nucleoprotein synthesis. Nature 163, 676–677 (1949)
Borgaonkar, D. S., Hollander, D. H.: Quinacrine fluorescence of the human Y chromosome. Nature 230, 52 (1971)
Caspersson, T., Zech, L., Johansson, C., Modest, E. J.: Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma (Berl.) 30, 215–227 (1970)
Dallapiccola, B.: Identification of the human sex chromosome complement in polymorphonuclear leukocytes: a new technique. J. Lab. Clin. Med. 78, 88–93 (1971)
Davidson, W. M., Smith, R.: A morphological sex difference in the polymorphonuclear neutrophil leukocytes. Br. Med. J. 2, 6–7 (1954)
George, K. P.: Cytochemical differentiation along human chromosomes. Nature 226, 80–81 (1970)
Goday, C., Egozcueuy, J., Bafall, E.: Sex chromatin fluorescence in hair root cells. Lancet 24, 220 (1971)
Hodgman, C. D., Weast, R. C., Selby, S. M.: Handbook of chemistry and physics. 40th. end., p. 1715. Cleveland, Ohio: Chemical Rubber Publ. Co. 1958
Pearson, P. L., Bobrow, M., Vosa, C. G.: Technique for identifying Y chromosomes in human interphase nuclei. Nature 226, 78–80 (1970)
Polani, P. E., Mutton, D. E.: Y-fluorescence of interphase nuclei, specially circulating lymphocytes. Br. Med. J. 1, 138–142 (1971)
Robinson, J.: Y chromosome fluorescence in buccal mucosa cells: a study on a new born population. Ann. Hum. Genet. 35, 61–65 (1971)
Rook, A., Hsu, L. Y., Gertner, M., Hirschhorn, K.: Idendification of Y and X chromosomes in amniotic fluid cells. Nature 230, 53 (1971)
Srivastava, P. K., Lucas, F. V.: Pattern of Y-chromatin fluorescence in an XYY man. Int. J. Clin. Genet. 6, 197–200 (1974)
Wyandt, H. E., Hecht, F.: Human Y-chromatin. 1. Dispersion and condensation. Exp. Cell Res 81, 453–461 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Siddiqui, A.H., Janssen de Limpens, A.M.P. & Cormane, R.H. Specific Y chromoome fluorescence in interphase nuclei of epithelial cells in human buccal smears. Arch Dermatol Res 262, 229–233 (1978). https://doi.org/10.1007/BF00455394
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00455394