Summary
In order to elucidate the mechanism underlying deposition of the substance observed mainly in the intercellular space of parakeratotic psoriatic horny layer, 6 cases of psoriasis vulgaris and, in comparison with these, 3 cases of pemphigus vulgaris were examined by using direct immunofluorescence technique with or without pretreatment with acid citrate buffer (pH 3.0) or digestion by fibrinolysin. The substance in the intercellular space of parakeratotic psoriatic horny layer showed positive fluorescence for IgG, IgA, IgM, C3 and fibrinogen. Positive fluorescence for IgG and fibrinogen was not significantly affected by preincubation of tissue sections in acid citrate buffer, but almost completely disappeared by digestion with fibrinolysin. On the contrary, positive fluorescence for IgG in the intercellular space of the prickle cell layer of pemphigus skin lesions almost completely disappeared by acid citrate buffer, but was not significantly affected by fibrinolysin. From these findings, it was suggested that the deposition of the substance in the intercellular space of parakeratotic psoriatic horny layer is rather the result of coagulation of plasma protein than that of antigen-antibody reactions.
Zusammenfassung
Um den Charakter der meistens in den intercellulären Räumen der parakeratotischen psoriatischen Hornschicht beobachteten Substanzen zu definieren wurden 6 Fälle von Psoriasis vulgaris und, im Vergleich mit ihnen, 3 Fälle von Pemphigus vulgaris untersucht mittels direkten Immunofluorescenz-Techniken mit oder ohne Vorbehandlung mit Zitronensäurenpufferlösungen (pH 3.0) oder Fibrinolysin. Die Substanz bei Psoriasis zeigte positive Immunofluorescenzen für IgG, IgA, IgM, C3 und Fibrinogen. Diese positive Fluorescenz für IgG und Fibrinogen wurde durch Vorbehandlungen mit Zitronensäurenpufferlösungen nicht wesentlich beeinflußt, verschwand aber fast vollständig durch Vorbehandlungen mit Fibrinolysinen. Im Vergleich mit diesen Befunden, verschwand die positive Fluorescenz für IgG in den intercellulären Räumen des Stratum spinosum von Pemphigushaut durch Vorbehandlungen mit Zitronensäurenpufferlösungen fast vollständig; Vorbehandlungen mit Fibrinolysinen war weniger wirksam. Aus diesen Befunden wurde geschlossen, daß die Deposition der Substanzen bei der Psoriasis mehr das Resultat einer Coagulation des Plasmaeiweiß sind, als daß diese sich aus Antigen-Antikörper-Reaktionen ableiten lassen.
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We thank Prof. H. Hatano and Ass. Prof. M. Nagashima for their review of the manuscript
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Kimura, S., Nishikawa, T. An immunohistochemical analysis of the deposited immunoglobulins or fibrinogen in parakeratotic psoriatic horny layer and pemphigus skin lesions. Arch Dermatol Res 261, 55–62 (1978). https://doi.org/10.1007/BF00455375
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DOI: https://doi.org/10.1007/BF00455375
Key words
- Psoriasis vulgaris
- Pemphigus vulgaris
- Direct immunofluorescence technique
- Acid citrate buffer
- Fibrinolysin