Abstract
The activities of ribulose 1,5-bisphosphate carboxylase and of carbonic anhydrase were studied in cell-free extracts of two symbiotic Chlorella strains isolated from Paramecium bursaria and from Spongilla sp., and of two nonsymbiotic strains of Chlorella (Chlorella fusca and Chlorella vulgaris) cultivated at varied CO2-concentrations. The symbiotic Chlorella of Paramecium bursaria differs distinctly from the other Chlorella strains by a higher activity of ribulose 1,5-bisphosphate carboxylase, which is independent of the actual CO2-concentration, and by a lack of carbonic anhydrase activity. These properties are discussed with respect to their ecological significance.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- CA:
-
carbonic anhydrase
- Pbi:
-
Paramecium bursaria isolate
- RuBP:
-
ribulose 1,5-bisphosphate
References
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Döhler G (1974a) Einfluß der Kulturbedingungen auf die photosynthetischen Carboxylierungsreaktionen von Chlorella vulgaris. Z Pflanzenphysiol 71:144–153
Döhler G (1974b) Photosynthesische Carboxylierungsreaktion synchron kultivierter Chlorella vulgaris. Z Pflanzenphysiol 73:283–295
Hogetsu D, Miyachi S (1979) Role of carbonic anhydrase in photosynthetic CO2 fixation in Chlorella. Plant and Cell Physiol 20:747–756
Karakashian SJ, Karakashian MW, Rudzinska MA (1968) Electron microscopic observations on the symbiosis of Paramecium bursaria and its intracellular algae. J Protozool 15:113–128
Nelson EB, Cenedella A, Tolbert NE (1969) Carbonic anhydrase levels in Chlamydomonas. Phytochemistry 8:2305–2306
Reed ML, Graham D (1977) Carbon dioxide and the regulation of photosynthesis: activities of photosynthetic enzymes and carbonate dehydratase (carbonic anhydrase) in Chlorella after growth or adaption in different carbon dioxide concentrations. Aust J Plant Physiol 4:87–98
Reisser W (1975) Zur Taxonomie einer auxotrophen Chlorella aus Paramecium bursaria. Arch Microbiol 104:293–295
Reisser W (1976) Die stoffwechselphysiologischen Beziehungen zwischen Paramecium bursaria Ehrbg. und Chlorella spec. in der Paramecium bursaria-Symbiose. II. Symbiose-spezifische Merkmale der Stoffwechselphysiologie und der Cytologie des Symbioseverbandes und ihre Regulation. Arch Microbiol 111:161–170
Reisser W (1980) The metabolic interactions between Paramecium bursaria Ehrbg. and Chlorella spec. in the Paramecium bursaria symbiosis. III. The influence of different CO2-concentrations and of glucose on the photosynthetic and respiratory capacity of the symbiotic unit. Arch Microbiol 125:291–293
Rickli EE, Ghanzanfar AS, Gibbons BH, Edsall JT (1964) Carbonic anhydrases from human erythrocytes. J Biol Chem 239:1065–1078
Zamenhof S, Eichhorn HH (1967) Study of microbial evolution through loss of biosynthetic functions: Establishment of “defective” mutants. Nature 216:456–458
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Dr. André Pirson on the occasion of his 70th birthday
Rights and permissions
About this article
Cite this article
Reisser, W., Benseler, W. Comparative studies on photosynthetic enzymes of the symbiotic Chlorella from Paramecium bursaria and other symbiotic and non-symbiotic Chlorella strains. Arch. Microbiol. 129, 178–180 (1981). https://doi.org/10.1007/BF00455358
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00455358