Archives of oto-rhino-laryngology

, Volume 244, Issue 4, pp 229–235 | Cite as

A scanning electron microscopic study of vestibular organ malformation following prenatal gamma irradiation

  • M. Hultcrantz
Article
  • 25 Downloads

Summary

Pregnant CBA/CBA mice were exposed to 1 and 2 Gy whole-body gamma irradiation on the 13th and 16th gestational days, respectively. The litters were born on the 21st day of gestation and were tested for vestibular function at the age of 1 month. The animals were then sacrificed and their inner ears were analyzed by scanning electron microscopy. No disturbances of vestibular function were noted in the animals studied. However, the cristae ampullares showed severe malformations as regards their gross shape, with irregularities of their outer contours. Type I hair cells seemed to be more severely changed than Type II hair cells, with fusion of sensory hairs, giant hair formation and bulging of the cuticular plate. In certain sites the hair cells were totally missing. These derangements were usually located in the central areas of the cristae ampullares and in the striolar portion of the maculae utriculi. The morphological damage found showed a dose-dependent, time-related pattern.

Key words

Inner ear morphology Embryology Irradiation Hair cells Vestibular organs 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anniko M, Hultcrantz M (1984) Vestibular hair cell pathology following low-dose irradiation during embryonic development. Acta Otolaryngol (Stockh) 98:292–301Google Scholar
  2. 2.
    Anniko M, Sobin A, Wersäll J (1980) Vestibular hair cell pathology in the Shaker-2 mouse. Arch Otorhinolaryngol 226:45–50Google Scholar
  3. 3.
    Anniko M, Nordemar H, Sobin A (1983) Principles in embryonic development and differentiation of vestibular hair cells. Otolaryngol Head Neck Surg 91:540–554Google Scholar
  4. 4.
    Anniko M, Lim DJ, Sobin A, Wroblewski R (1985) Applications of SEM and SEM-X-ray microanalysis in inner ear pathology. Scanning Electron Microscopy 1:345–355Google Scholar
  5. 5.
    Aursnes J (1980) Vestibular damage from chlorhexidine in guinea pigs. Acta Otolaryngol (Stockh) 92:89–100Google Scholar
  6. 6.
    Ernstson S, Lundquist P-G, Wedenberg E, Wersäll J (1969) Morphological changes in vestibular hair cells in a strain of the waltzing guinea pig. Acta Otolaryngol (Stockh) 67:521–534Google Scholar
  7. 7.
    Hicks SP (1953) Developmental malformations produced by radiation. Am J Roentgenol 69:272–292Google Scholar
  8. 8.
    Hirokawa N, Tilney LG (1982) Interactions between actin filaments and between actin filaments and membranes in quick-frozen and deeply etched hair cells of the chick ear. J Cell Biol 95:249–261Google Scholar
  9. 9.
    Hultcrantz M, Anniko M (1984) Malformations of the vestibular organs following low-dose gamma irradiation during the embryonic development. Acta Otolaryngol (Stockh) 97:7–17Google Scholar
  10. 10.
    Hultcrantz M, Anniko M (1985) Influence of gamma irradiation on developing otoconia. Am J Otolaryngol 6:79–91Google Scholar
  11. 11.
    Hultcrantz M, Anniko M, Borg E (1985) Structure and function of the adult cochlea following prenatal irradiation. Acta Otolaryngol (Stockh) [Suppl] 425:1–31Google Scholar
  12. 12.
    Hunter-Duvar IM (1978) Electron microscopic assessment of the cochlea. Some techniques and results. Acta Otolaryngol (Stockh) [suppl] 351:1–44Google Scholar
  13. 13.
    Job T, Liebold GJ, Fitzmaurice HA (1935) Biological effects of roentgen rays. Am J Anat 56:97–117Google Scholar
  14. 14.
    Lim DJ, Anniko M (1985) Developmental morphology of the mouse inner ear. A scanning electron microscopic observation. Acta Otolaryngol (Stockh) [Suppl] 422:1–69Google Scholar
  15. 15.
    Lyon M (1951) Hereditary absence of otoliths in the house mouse. J Physiol (Lond) 114:410–418Google Scholar
  16. 16.
    Michel C, Fritz-Niggli H (1978) Radiation-induced developmental anomalies in mammalian embryos by low doses and interaction with drugs, stress and genetic factors. In: Late biological effects of ionizing radiation, vol 2. International Anatomic Energy Agency, Vienna, pp 397–408Google Scholar
  17. 17.
    Nordemar H (1983) Postnatal development of the vestibular sensory epithelium in the mouse. Acta Otolaryngol (Stockh) 96:447–456Google Scholar
  18. 18.
    Ruben RJ (1967) Development of the inner ear of the mouse: A radioautographic study of the terminal mitosis. Acta Otolaryngol (Stockh) [Suppl] 220:1–44Google Scholar
  19. 19.
    Russel LR (1950) X-ray induced developmental abnormalities in the mouse and their use in the analyzis of embryonic patterns. J Exp Zool 114:545–601Google Scholar
  20. 20.
    Sobin A, Wersäll J (1983) A morphological study on vestibular sensory epithelia in the waltzing guinea pig. Acta Otolaryngol (Stockh) [Suppl] 396:1–32Google Scholar
  21. 21.
    Wersäll J, Björkroth B, Flock Å, Lundquist P-G (1971) Sensory hair fusion in vestibular sensory cells after gentamycin exposure. A transmission and scanning electron microscopic study. Arch Klin Exp Ohr-, Nas-, Kehlk-Heilkd 200:1–14Google Scholar
  22. 22.
    Wersäll J, Anniko M, Bagger-Sjöbäck D, Lundquist P-G, Sobin A (1979) Feinstrukturelle Veränderungen des vestibulären Organs unter funktionellen und toxischem Einfluß. In: Berends J, Link R, Zöllner F (Hrsg) Hals-, Nasen- u Ohren-Heilkunde, vol 5/I. Thieme, Stuttgart, pp 7.1–7.23Google Scholar
  23. 23.
    Winther FÖ (1970) X-ray irradiation of the inner ear of the guinea pig. Thesis, University of OsloGoogle Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. Hultcrantz
    • 1
  1. 1.Department of OtolaryngologyKarolinska HospitalStockholmSweden

Personalised recommendations