Summary
Radioactive lanthanum nitrate, an electron opaque tracer, was injected into the common bile duct of rats. Two minutes following the end of injection, samples of blood for radioactivity counts, and of liver and kidney for electron microscopic studies were taken. High levels of radioactivity found in the blood, and demonstration of lanthanum in the kidney by electron microscopy, indicated that this tracer entered the blood stream in vivo. No lanthanum was seen in the cytoplasm of liver cells, and there was no evidence of rupture in bile ducts, junctional ducts, or liver cells. Tight junctions connecting parenchymal cells and cholangiolar cells appeared well preserved. Lanthanum was seen in bile canaliculi, interspaces between liver cells, portions of the extracellular space of the liver, lumina of cholangioles and lumina of portal venules and sinusoids. It is postulated that lanthanum passed from the biliary tract, the site of injection, through the tight junction between liver cells and cholangiolar cells. It is suggested that such passage may represent a physiologic pathway, but the possibility of a chemical action of lanthanum on the tight junction can not be ruled out.
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References
Berlin, N., Huff, R., Dyke, D. van, Hennessy, T.: The blood volume of the adult rat as determined by Fe59 and P32 labeled red cells. Proc. Soc. exp. Biol. (N.Y.) 71, 176–178 (1949).
Braver, R.: Liver circulation and function. Physiol. Rev. 43, 115–213 (1963).
Bulger, R., Trump, B.: Retrograde injection of thorotrast, ferritin and india ink into flounder urinary tract. J. Morph. 127, 205–224 (1969).
Bullivant, S., Loewenstein, W.: Structure of coupled and uncoupled cell junctions. J. Cell Biol. 37, 621–632 (1968).
DePalma, R., Vogt, C., Wilburn, J., Holden, W.: An electron microscopic study of extra-hepatic biliary obstruction: route to entry of bile into the blood stream. J. Amer. med. Ass. 204, 534 (1968).
Division of Radiological Health, U. S. Department of Health, Education and Welfare, Radiological Health Handbook, Washington, D. C., p. 338, 1962.
Dzhelegov, B., Prikhodetseva, V., Khol'nov, Y.: Isobaric nuclei with the mass number A=140, as quoted in Division of Technical Information, United States Atomic Energy Commission. Nuclear Science Abstracts, No 31125, 16, 4092 (1962).
Farquhar, M., Palade, G.: Junctional complexes in various epithelia. J. Cell Biol. 17, 375–412 (1963).
Goodenough, D., Revel, J.: A fine structural analysis of intercellular junctions in the mouse liver. J. Cell Biol. 45, 273–290 (1970).
Huang, K., Bondurant, J.: Simultaneous estimation of plasma volume, red cell volume and thiocyanate space in unanesthesized normal and splenectomized rats. Amer. J. Physiol. 185, 441–445 (1956).
Matter, A., Orci, L., Rouiller, C.: A study on the permeability barriers between Disse's space and the bile canaliculus. J. Ultrastruct. Res., Suppl. 11 (1969).
McNutt, N., Weinstein, R.: The ultrastructure of the nexus. J. Cell Biol. 47, 666–688 (1970).
Montgomery, P.: A method for determining blood volume of the rat using radioactive phosphorus. Proc. Soc. exp. Biol. (N. Y.) 77, 445–447 (1951).
Papadimitriou, J., Walters, M.: Fluid flow in the liver demonstrated with horse radish peroxidase. Amer. J. Anat. 123, 475–488 (1968).
Popper, H., Schaffner, F.: Pathophysiology of cholestasis. Human Pathology 1, 1–24 (1970).
Revel, J., Karnovsky, M.: Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33, C7-C12 (1967).
Rothman, A.: Alcian blue as an electron stain. Exp. Cell Res. 58, 177–179 (1969).
Sasaki, H., Schaffner, F., Popper, H.: Bile ductules in cholestasis: morphologic evidence for secretion and absorption in man. Lab. Invest. 16, 84–95 (1967).
Schatzki, P.: Bile canaliculus and space of Disse: electron microscopic relationships as delineated by lanthanum. Lab. Invest. 20, 87–93 (1969).
Shea, S., Karnovsky, M.: The cell surface and intercellular junctions in liver as revealed by lanthanum staining after fixation with glutaraldehyde with added alcian blue. J. Cell Biol. 43, 128a-129a (1969).
Spycher, M.: Intercellular adhesions. Z. Zellforsch. 111, 64–74 (1970).
Trowell, O.: The experimental production of watery vacuoles of the liver. J. Physiol. (Lond.) 105, 268–297 (1946).
Wisse, E.: An electron microscopic study of the fenestrated endothelial lining of the rat liver sinusoid. J. Ultrastruct. Res. 31, 125–150 (1970).
Yodaiken, R.: The use of lead as a tracer in ultrastructural research. Lab. Invest. 15, 403–411 (1966).
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This study was supported by the A. D. Williams Fund of Virginia Commonwealth University and the U. S. Veterans Administration. Radiation Counting Equipment was loaned by Dr. F. O'Foghludha, Department of Radiation Physics, Virginia Commonwealth University.
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Schatzki, P.F. The passage of radioactive lanthanum from the biliary to the vascular system. Z.Zellforsch 119, 451–459 (1971). https://doi.org/10.1007/BF00455242
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DOI: https://doi.org/10.1007/BF00455242