Effect of short physical exercise on the levels of zinc and carbonic anhydrase isoenzyme activities in human erythrocytes

  • H. Ohno
  • F. Hirata
  • K. Terayama
  • T. Kawarabayashi
  • R. Doi
  • T. Kondo
  • N. Taniguchi
Article

Summary

Studies of the influences of physical exercise of short duration (bicycle ergometer, 200 W for 30 min) on the activities of carbonic anhydrase isoenzymes (CA-B and CA-C types) and zinc concentration in erythrocytes were made on 5 untrained healthy male volunteers. The subjects were rested for 30 min after the exercise. There were significant decreases in the levels of zinc, CA-B, total carbonic anhydrase activity and CA-B-dependent activity immediately after exercise, but after 30 min of rest they all returned to their pre-exercise levels. No significant change in CA-C level or CA-C-dependent activity was found after exercise. Immediately after exercise, total carbonic anhydrase activity and CA-B-dependent activity following the addition of Zn2+ showed significant increases, compared with their respective activities without Zn2+ addition. However, no such effects were observed just before exercise or after rest; the addition of Zn2+ had no effect on CA-C-dependent activity at any time. A significant correlation was found between the changes in concentration of zinc and CA-B-dependent activity after exercise (r=0.711). The findings of the present study suggest that active CA-B enzymes are converted in part to inactive enzymes during acute physical exercise, possibly by decreased zinc binding. Moreover, the change in CA-B-dependent activity correlated well with the changes in pH and HCO3 concentrations in venous blood (r=0.853 and r=0.718, respectively). One may speculate that an adaptive decrease in CA-B-dependent activity in erythrocytes occurs with increased acidification in blood during heavy physical exercise of short duration.

Key words

Carbonic anhydrase isoenzyme Zinc pH Erythrocyte Exercise 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • H. Ohno
    • 1
  • F. Hirata
    • 1
  • K. Terayama
    • 1
  • T. Kawarabayashi
    • 1
  • R. Doi
    • 2
  • T. Kondo
    • 3
  • N. Taniguchi
    • 3
  1. 1.Department of Hygiene and Preventive MedicineAsahikawa Medical CollegeAsahikawaJapan
  2. 2.Department of Public HealthAsahikawa Medical CollegeAsahikawaJapan
  3. 3.Biochemistry Laboratory, Cancer InstituteHokkaido University School of MedicineSapporoJapan

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