Summary
In a search for an experimental procedure to synchronize meiosis in fruit bodies of Schizophyllum commune, the effect of hydroxyurea on sporulation was tested. Results indicate that hydroxyurea has an immediate and reversible effect on basidiospore sporulation, germination, and nuclear number. A tentative time schedule for meiosis is presented.
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Carmi, P., Koltin, Y., Stamberg, J.: Meiotic synchrony in Schizophyllum commune induced with hydroxyurea. (Submitted for publication)
Chiu, S.M., Hastings, P.J.: Premeiotic DNA synthesis and recombination in Chlamydomonas reinhardi. Genetics 73, 29–43 (1973)
Hotta, Y., Stern, H.: Analysis of DNA synthesis during meiotic prophase in Lilium. J. molec. Biol. 55, 337–355 (1971)
Hundert, P., Koltin, Y., Stamberg, J.: Repair of UV-induced damage in Schizophyllum commune. Genetics (in press)
Koltin, Y., Stamberg, J.: Genetic control of recombination in Schizophyllum commune: Location of a gene controlling β-factor recombination. Genetics 74, 55–62 (1973)
Koltin, Y., Stamberg, J., Ronen, R.: Meiosis as a source of spontaneous mutations in Schizophyllum commune. Mutation Res. 27, 319–325 (1975)
Lu, B.C., Jeng, D.Y.: Meiosis in Coprinus: VII. The prekaryogamy S-phase and the postkaryogamy DNA replication in C. lagopus. J. Cell Sci. 17, 461–470 (1975)
Radu, M., Steinlauf, R., Koltin, Y.: Meiosis in Schizophyllum commune: Chromosomal behavior and the synaptinemal complex. Arch. Microbiol. 98, 301–310 (1974)
Raju, N.B., Lu, B.C.: Meiosis in Coprinus: III. Timing of meiotic events in C. lagopus (sensu Buller). Canad. J. Bot. 48, 2183–2186 (1970)
Raudaskoski, M.: Light and electron microscope study of unilateral mating between a secondary mutant and a wildtype strain of Schizophyllum commune. Protoplasma 76, 35–48 (1973)
Schwalb, M.N.: Commitment to fruiting in synchronously developing cultures of the basidiomycete Schizophyllum commune. Arch. Microbiol. 79, 102–107 (1971)
Simchen, G., Idar, D., Kassir, Y.: Recombination and hydroxyuerea inhibition of DNA synthesis in yeast meiosis. Molec. gen. Genet. 144, 21–27 (1976)
Simchen, G., Stamberg, J.: Fine and coarse controls of genetic recombination. Nature (Lond.) 222, 329–332 (1969a)
Simchen, G., Stamberg, J.: Genetic control of recombination in Schizophyllum commune: Specific and independent regulation of adjacent and non-adjacent chromosomal regions. Heredity 24, 369–381 (1969b)
Slater, M.L.: Effect of reversible inhibition of deoxyribonucleic acid synthesis on the yeast cell cycle. J. Bact. 113, 263–270 (1973)
Stamberg, J., Koltin, Y.: Genetic control of recombination in Schizophyllum commune: Evidence for a new type of regulatory site. Genet. Res. (Camb.) 22, 101–111 (1973)
Stevens, R.B.: Mycology Guidebook, 703 pp. Seattle and London: University of Washington Press, 1974
Timson, J.: Hydroxyurea. Mutation Res. 32, 115–132 (1975)
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Communicated by F. Kaudewitz
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Carmi, P., Raudaskoski, M., Stamberg, J. et al. Meiosis in Schizophyllum commune: The effect of hydroxyurea on basidiospore sporulation, germination, and nuclear number. Molec. Gen. Genet. 158, 17–21 (1977). https://doi.org/10.1007/BF00455115
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DOI: https://doi.org/10.1007/BF00455115