Molecular and General Genetics MGG

, Volume 158, Issue 1, pp 11–15 | Cite as

Inhibition of thymidine phosphorylase in vivo provides a rapid method for switching DNA labeling

  • John E. Womack


Uridine blocks the in vivo conversion of thymine to thymidine in Escherichia coli, thus, one can change DNA labels by labelling first with a thymine label (e.g. 14C) and then, at the time of the change, adding 50 μg uridine per ml and thymidine (e.g. 3H). The cells immediately start using the thymidine, ignore the thymine for several generations, and are not affected by the uridine.


Escherichia Coli Thymidine Uridine Thymine Rapid Method 
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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • John E. Womack
    • 1
  1. 1.Abt. TrautnerMax-Planck-Institut für molekulare GenetikBerlin 33

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