Abstract
In vitro autoradiography with tritiated thymidine was performed in guinea pig cornea and guinea pig skin after in vitro exposure to solar simulating radiation (UVB). UVA-irradiated and unirradiated specimens served as controls. Sparsely labelled nuclei, indicating unscheduled thymine dirtier repair DNA-synthesis (dark repair), were observed immediately after UVB exposure in the epidermis and upper dermis of the skin and in all cellular compartments of the cornea. Control samples did not exhibit dark repair. The ratio of sparsely labelled cells was similar in the epidermis and in the corneal epithelium, but was significantly higher in the corneal stroma and highest in the endothelium.
Zusammenfassung
Hornhaut und Haut von Meerschweinchen wurde nach in vitro-Bestrahlung mit einem Sonnensimulator (UVB) mittels Autoradiographie unter Verwendung von Tritium-markiertem Thymidin in vitro untersucht.
UVA-exponierte und unbestrahlte Proben dienten als Kontrollen. Lokkere autoradiographische Markierung, ein Marker der unprogrammierten DNS-Reparatur-Synthese, fand sich sofort nach UVB-Exposition über Zellkernen der Epidermis und oberen Dermis der Haut, sowie in allen zellulären Schichten der Hornhaut. In Kontrollpräparaten fand sich keine lockere Markierung. Der Anteil locker markierter Zellkerne in der Epidermis und im Hornhautepithel war nahezu gleich, während sich im Hornhautstroma und besonders im Endothel eine signifikant höhere Anzahl an Zellen in DNS-Reparatur-Synthese befanden.
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Brenner, W., Grabner, G. 3H-thymidine autoradiography of guinea pig cornea and skin after exposure to solar simulating radiation. Albrecht von Graefes Arch. Klin. Ophthalmol. 216, 319–325 (1981). https://doi.org/10.1007/BF00455039
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DOI: https://doi.org/10.1007/BF00455039