Archives of Microbiology

, Volume 144, Issue 1, pp 62–66 | Cite as

Glucose as an energy donor in acetate growing Acinetobacter calcoaceticus

  • Roland H. Müller
  • Wolfgang Babel
Original Papers

Abstract

Since glucose can be oxidized but not assimilated by Acinetobacter calcoaceticus 69-V the question arose whether energy generated by glucose oxidation can help incorporate carbon from heterotrophic substrates and, if so, what the efficiency of ATP production is like. For this reason this species was grown in the chemostat on acetate. After having reached steady state conditions an increasing concentration of glucose was added. This led to an increase in the biomass level from about 0.4 g/g for growth on acetate alone to 0.6–0.65 g/g in the presence of glucose, independently of either the growth rate or the steepness of the glucose gradient used. This upper value approximates about the limit of the carbon conversion efficiency calculated for non-glycolytic substrates. Glucose was almost exclusively oxidized to gluconic acid, 2- and 5-ketogluconates, and pentose 5-phosphates were found only in traces. These results demonstrate that glucose functions as an additional energy source in Acinetobacter calcoaceticus 69-V. From the transient behaviour of biomass increase and the mixing proportion at which the maximum growth yield on acetate in the presence of glucose was obtained it followed that two mol of ATP must have been generated per mol of glucose oxidized. This property is discussed in terms of coupling glucose dehydrogenase with the respiratory chain.

Key words

Acinetobacter calcoaceticus Continuous cultivation Acetate growth Glucose oxidation Energy gain Maximum carbon conversion efficiency 

Abbreviations

Gox

glucose oxidized to gluconic acid

Gt

amount of glucose necessary for complete substitution of Sd

So

inlet concentration of the limiting carbon substrate

Sa and Sd

assimilated and dissimilated part respectively of the carbon substrate

PQQ

pyrrolo-quinoline-quinone

VATPAc

ATP gain from complete oxidation to CO2 of acetate (P/O=2)

VATPGlc

ATP gain from oxidation of glucose to gluconic acid

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Roland H. Müller
    • 1
  • Wolfgang Babel
    • 1
  1. 1.Institute of BiotechnologyAcademy of Sciences of the GDRLeipzigGerman Democratic Republic

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