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Archives of Microbiology

, Volume 144, Issue 1, pp 20–24 | Cite as

Antigenic determinants distinctive of Methanospirillum hungatei and Methanogenium cariaci identified by monoclonal antibodies

  • E. Conway de Macario
  • H. König
  • A. J. L. Macario
Original Papers

Abstract

Monoclonal antibodies were prepared against two species of Methanomicrobiaceae. Antibody 1A is specific for Methanospirillum hungatei strain JF1 and the determinant it recognizes is expressed on the surface of JF1 cells, where it is exposed and accessible to antibody. The determinant is found in a polypeptide (MW<12,000) in the sheath that covers the bacterial cell; it is not present in Methanospirillum hungatei strain GP1; and it is not expressed on the surface of whole cells of the other 24 methanogenic bacteria tested. It is therefore a marker of strain JF1, consequently, antibody 1A is potentially useful for tracking JF1 and fragments thereof in a variety of samples. Antibody 7A is specific for Methanogenium cariaci JR1c. It did not react with any other methanogen tested, not even with Mg. marisnigri or Ms. hungatei JF1, although these cross-react with Mg. cariaci if tested with polyclonal antisera. Therefore antibody 7A recognizes specifically a marker of Mg. cariaci JR1c.

Key words

Methanospirillum hungatei Methanogenium cariaci Monoclonal antibodies Distinctive antigenic determinants JF1 strain sheath JR1c strain S layer Slide immunoenzymatic assay 

Abbreviations

SIA

slide immunoenzymatic assay

SDS-PAGE

sodium dodecylsulfate polyacrylamide gel electrophoresis

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • E. Conway de Macario
    • 1
  • H. König
    • 2
  • A. J. L. Macario
    • 1
  1. 1.Wadsworth Center for Laboratories and ResearchNew York State Department of HealthAlbanyUSA
  2. 2.Lehrstuhl für Mikrobiologie der Universität RegensburgRegensburgGermany

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