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Eubacterium oxidoreducens sp. nov. requiring H2 or formate to degrade gallate, pyrogallol, phloroglucinol and quercetin

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Abstract

Based on most probable number (MPN) estimates of rumen fluid from a hay-fed steer, 10 mM gallate was decarboxylated by 9.3×106 bacteria per ml. It was decarboxylated and reductively dehydroxylated by 9.3×105 bacteria per ml and was further catabolized to non-aromatic products by 4.3×103 bacteria per ml. Resorcinol was not further degraded and, with 0.1 ml of inoculum, catechol was not degraded. Strain G41 was isolated from a pyrogallolmedium roll tube inoculated with 1 μl of rumen fluid and, with slight modifications of the generic description, was named Eubacterium oxidoreducens sp. nov. It was an anaerobic, nonmotile, curved, Gram-positive, small rod with rounded ends and required H2 or formate to degrade gallate, pyrogallol, phloroglucinol or quercetin to acetate and butyrate and, in the case of quercetin, to 3,4-dihydroxyphenylacetate. Crotonate was catabolized to acetate and butyrate and no electron donor was required. No other compounds were degraded with or without an electron donor or with Desulfovibrio sp. plus sulfate as a possible electron acceptor system. E. oxidoreducens grew well in a chemically-defined culture medium containing usable energy source, minerals, B-vitamins, cysteine and CO2−HCO 3- -buffer, pH 7.2.

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Abbreviations

G+C:

Guanine plus cytosine

MPN:

most probable number

OD:

optical density

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Department of Dairy Science, University of Illinois, 61801, Urbana, IL, USA

    Lee R. Krumholz & M. P. Bryant

  2. Department of Microbiology, University of Illinois, 61801, Urbana, IL, USA

    M. P. Bryant

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  1. Lee R. Krumholz
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  2. M. P. Bryant
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Krumholz, L.R., Bryant, M.P. Eubacterium oxidoreducens sp. nov. requiring H2 or formate to degrade gallate, pyrogallol, phloroglucinol and quercetin. Arch. Microbiol. 144, 8–14 (1986). https://doi.org/10.1007/BF00454948

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  • DOI: https://doi.org/10.1007/BF00454948

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