Advertisement

Archives of Microbiology

, Volume 144, Issue 1, pp 1–7 | Cite as

Nitrospira marina gen. nov. sp. nov.: a chemolithotrophic nitrite-oxidizing bacterium

  • Stanley W. Watson
  • Eberhard Bock
  • Frederica W. Valois
  • John B. Waterbury
  • Ursula Schlosser
Original Papers

Abstract

A new chemolithotrophic nitrite-oxidizing bacterium, for which the name Nitrospira marina is proposed, was isolated from the Gulf of Maine. N. marina is a Gramnegative curved rod which may form spirals with 1 to 12 turns. Cells have a unique periplasmic space and lack intracytoplasmic membranes and carboxysomes. N. marina is an obligate chemolithotroph, but best growth is obtained in a mixotrophic medium. N. marina may be one of the most prevalent nitrite-oxidizing bacteria in some oceanic environments. Type strain is field with American Type Culture Collection (ATCC 43039).

Key words

Nitrospira marina Nitrite-oxidizing bacterium Chemolithotrophic Mixotrophic Periplasmic space 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barnes H (1959) Apparatus and methods of oceanography. George Allen & Unwin Ltd, London, pp 117–118Google Scholar
  2. Bendschneider K, Robinson RJ (1952) A new spectrophotometric method for the determination of nitrite in sea water. J Mar Res 11:87–96Google Scholar
  3. Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Ann Biochem 72:248–254Google Scholar
  4. De Ley J (1970) Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101:738–754Google Scholar
  5. Heubült J (1929) Untersuchungen über Nitritbakterien. Planta 8:398–422Google Scholar
  6. Kraft I, Bock E (1984) Plasmids in Nitrobacter. Arch Microbiol 140:79–82Google Scholar
  7. Marmur Y (1961) A procedure for the isolation of desoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218Google Scholar
  8. Milde K, Bock E (1984) Isolation and partial characterization of inner and outer membrane fractions of Nitrobacter hamburgensis. FEMS Microbiol Lett 21:137–141Google Scholar
  9. Murray RGE, Watson SW (1965) Structure of Nitrosocystis oceanus and comparison with Nitrosomonas and Nitrobacter. J Bacteriol 89:1594–1609Google Scholar
  10. Spector T (1978) Refinement of the coomassie blue method of protein quantitation. Ann Biochem 86:142–146Google Scholar
  11. Sundermeyer-Klinger H, Meyer W, Warninghoff B, Bock E (1984) Membrane-bound nitrite oxidoreductase of Nitrobacter: evidence for a nitrate reductase system. Arch Microbiol 140:153–158Google Scholar
  12. Watson SW (1965) Characteristics of marine nitrifying bacterium, Nitrosocystis oceanus sp. n. Limnol Oceanogr (Suppl) 19: R274-R289Google Scholar
  13. Watson SW (1971) Reisolation of Nitrosospira briensis S. Winogradsky and H. Winogradsky 1933. Arch Microbiol 75:179–188Google Scholar
  14. Watson SW (1974) Family I. Nitrobacteraceae. In: Buchanan RE, Gibbons NE (ed) Bergey's manual of determinative bacteriology, 8th ed. The Williams & Wilkins Co, Baltimore, pp 450–456Google Scholar
  15. Watson SW, Graham LB, Remsen CC, Valois FW (1971) A lobular, ammonia-oxidizing bacterium: Nitrosolobus multiformis nov. gen. nov. sp. Arch Mikrobiol 76:183–203Google Scholar
  16. Watson SW, Waterbury, JB (1971) Characteristics of two marine nitrite oxidizing bacteria, Nitrospina gracilis nov. gen. nov. sp. and Nitrococcus mobilis nov. gen. nov. sp. Arch Mikrobiol 77:203–230.Google Scholar
  17. Winogradsky S (1892) Contribution à la morphologie des organismes de la nitrification. Arch Sci Biol (St Petersb) 1:86–137Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Stanley W. Watson
    • 1
  • Eberhard Bock
    • 2
  • Frederica W. Valois
    • 1
  • John B. Waterbury
    • 1
  • Ursula Schlosser
    • 2
  1. 1.Department of BiologyWoods Hole Oceanographic InstitutionWoods HoleUSA
  2. 2.Mikrobiologische AbteilungInstitut für Allgemeine Botanik der Universität HamburgHamburg 52Germany

Personalised recommendations