Archives of Microbiology

, Volume 140, Issue 2–3, pp 193–197 | Cite as

Metabolism of methylammonium by Azotobacter vinelandii

  • Richard A. Moore
  • Joyce K. Gordon
Original Papers

Abstract

Azotobacter vinelandii takes up the ammonium analog methylammonium from the external medium and metabolizes it to a less polar compound which has been identified as N-methylglutamine. The enzyme glutamine synthetase appears responsible for methylammonium metabolism in this organism and full activity of the enzyme is required for maximal rates of methylammonium uptake. L-methionine-DL-sulfoximine or L-methionine sulfone, inhibitors of glutamine synthetase activity, were shown to reduce the rate of methylammonium uptake by wild type cultures. A mutant strain with low glutamine synthetase activity was shown to be unable to carry out in vitro N-methylglutamine synthesis or in vivo uptake of methylammonium. Thus, methylammonium uptake assays may prove useful as a method of identifying mutants with altered glutamine synthetase activity.

Key words

Methylammonium Glutamine synthetase N-methylglutamine Azotobacter vinelandii 

Abbreviations

MSX

L-methionine-DL-sulfoximine

MSF

L-methionine sulfone

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Richard A. Moore
    • 1
  • Joyce K. Gordon
    • 1
  1. 1.Department of MicrobiologyUniversity of British ColumbiaVancouverCanada

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