Abstract
Membranes prepared from Paracoccus halodenitrificans reduced nitrite or nitric oxide to nitrous oxide. Extraction of these membranes with the detergent CHAPSO [3-(3-cholamidopropyldimethylammonio)-1-(2-hydroxy-1-propanesulfonate)], followed by ammonium sulfate fractionation of the solubilized proteins, resulted in the separation of nitrite and nitric oxide reductase activities. The fraction containing nitrite reductase activity spectrally resembled a cd-type cytochrome. Several cytochromes were detected in the nitric oxide reductase fraction. Which, if any, of these cytochromes is associated with the reduction of nitric oxide is not clear at this time.
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Abbreviations
- PMS:
-
phenazine methosulfate
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- CHAPSO:
-
3-(3-cholamidopropyl-dimethylammonio)-1-(2-hydroxy-1-propanesulfonate)
- NH buffer:
-
150 mM NaCl-50 mM
- HEPES:
-
pH 7.5; octylglucoside, octyl-β-d glucopyranoside
- NIR:
-
intrite reductase (nitrite to nitric oxide)
- NOR:
-
nitric oxide reductase (nitric oxide to nitrous oxide)
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Grant, M.A., Cronin, S.E. & Hochstein, L.I. Solubilization and resolution of the membrane-bound nitrite reductase from Paracoccus halodenitrificans into nitrite and nitric oxide reductases. Arch. Microbiol. 140, 183–186 (1984). https://doi.org/10.1007/BF00454923
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DOI: https://doi.org/10.1007/BF00454923