Abstract
Lipid classes and fatty acid distribution were analysed in the resting sporangium of Synchytrium endobioticum, the causal agent of the potato wart disease. The sporangium contents were shown to have lipid droplets, the major fatty acids there being C16.0, C18.1, and C19.0. The sporangium wall on the other hand was composed of C18.0, C18.1, C18.2, C20.0, and C20.4 fatty acids. A significantly large portion of the sporangium wall lipids contained wax esters with branched chains.
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References
Ackman RG (1967) Calculations of ECL values in the gas liquid chromatography of multiple-branched fatty acids. J Chromatogr 28:225–231
Ackman RG (1968) Prediction of retention times in the gas liquid chromatography of disastereoisomers of methyl-branched fatty acids. J Chromatogr 34:165–173
Baker EA (1982) Chemistry and morphology of plant epicuticular waxes. In: Cutler DF, Alvin KL, Price CE (eds) The plant cuticle. Academic Press, London, pp 140–165
Bal AK, Murphy AM, Hampson MC (1981) Ultrastructure and chemical analysis of the resting sporangium wall Synchytrium endobioticum. Can J Plant Pathol 3:86–89
Bouligand Y (1965) Sur une architecture torsadee repondue dans de nombre uses cuticles d'arthropods. C. R. Hedd. Seanc Acad Sci (Paris) 262:3665–3668
Burnett JH (1968) Fundamentals of mycology. Edward Arnold Ltd, London, p 546
Chopra A, Khuller GK (1983) Lipids of pathogenic fungi. Prog Lipid Res 22:189–220
Christie WW (1976) The structure, chemistry and occurrence of lipids. In: Christie WE (ed) Lipid analysis. Pergamon Press, New York, pp 2–12
Dey AC, Abbott EC, Rusted IE, Senciall IR (1972) Excretion of conjugated 11-deoxy-17-ketosteroids in essential hypertension. Can J Biochem 50:1273–1281
Dey AC, Kiceniuk JW, Williams UP, Khan RA, Payne JF (1983) Longterm exposure of marine fish to crude petroleum. I. studies on liver lipids and fatty acids in cod (Gadus morhual) and winter flounder Pseudopleuronectes Americanus). Comp Biochem Physiol 75C:93–101
Gilby AR (1980) Transpiration, temperature and lipids in insect cuticle. In: Berridge MJ, Treherne JE, Wigglesworth VB (eds) Advances in insect physiology, vol 15. Academic Press, New York, pp 1–33
Goss JA (1973) Physiology of plants and their cells. Pergamon Press, New York
Hampson MC, Coombes JW, Murphy AM (1983) Procedure for isolating populations of whole and fragmented resting sporangia of Synchytrium endobioticum (Schilb) Perc. from potato wart tissue for electron microscopy and chemical analysis. Plant and Soil 72:49–55
Holloway PJ (1982) The chemical composition of plant cutins. In: Cutler DF, Alvin KL, Price CE (eds) The plant cuticle. Academic Press, London, pp 46–85
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137A
Lange L, Olson LW (1981) Germination and parasitation of the resting sporangium of Synchytrium endobioticum. Protoplasma 106:69–82
Locke M (1974) The structure and formation of the integument in insects. In: Rockstein M (ed) The physiology of insecta, vol 5. Academic Press, New York, pp 123–313
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Margold HK, Malins DC (1960) Fractionation of fats, oils and waxes in thin layers of silicic acid. J Am Oil Chem Soc 37:383–385
Murphy AM, Bal AK, Hampson MC (1981) Incorporation of uridine disphospho-N-acetyl-d-glucosamine in the resting sporangium wall of Synchytrium endobioticum. Experientia 38:244–245
Patel TR, Bal AK, Hampson MC (1984) Partial characterization of the cell wall protein of the resting sporangium of Synchytrium endobioticum. Can J Microbiol (in press)
Pratt MA (1979) Potato wart disease and its legislative control in England and Wales. In: Ebbels DL, King JE (eds) Plant health. Halsted Press, New York, pp 199–212
Spurr A (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Sussman AS, Halvorson HO (1966) Spores: their dormancy and germination. Harper and Row, New York, pp 260–264
Yatsu LY, Jacks TJ, Hensarling TP (1971) Isolation of spherosomes (oleosomes) from onion, cabbage and cotton seed tissues. Plant Physiol 48:675–682
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Bal, A.K., Dey, A.C. & Hampson, M.C. Resting sporangium of Synchytrium endobioticum: its structure and composition of the lipids and fatty acids. Arch. Microbiol. 140, 178–182 (1984). https://doi.org/10.1007/BF00454922
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DOI: https://doi.org/10.1007/BF00454922