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Archives of Microbiology

, Volume 151, Issue 6, pp 530–536 | Cite as

ATP is required for K+ active transport in the archaebacterium Haloferax volcanii

  • Jean Meury
  • Masamichi Kohiyama
Original Papers

Abstract

The Archaebacterium Haloferax volcanii concentrates K+ up to 3.6 M. This creates a very large K+ ion gradient of between 500- to 1,000-fold across the cell membrane. H. volcanii cells can be partially depleted of their internal K+ but the residual K+ concentration cannot be lowered below 1.5 M. In these conditions, the cells retain the ability to take up potassium from the medium and to restore a high internal K+ concentration (3 to 3.2 M) via an energy dependent, active transport mechanism with a Km of between 1 to 2 mM. The driving force for K+ transport has been explored. Internal K+ concentration is not in equilibrium with ΔΨm suggesting that K+ transport cannot be accounted for by a passive uniport process. A requirement for ATP has been found. Indeed, the depletion of the ATP pool by arsenate or the inhibition of ATP synthesis by N,N′-dicyclohexylcarbodiimide inhibits by 100% K+ transport even though membrane potential ΔΨm is maintained under these conditions. By contrast, the necessity of a ΔΨm for K+ accumulation has not yet been clearly demonstrated. K+ transport in H. volcanii can be compared with K+ transport via the Trk system in Escherichia coli.

Key words

Archaebacteria K+ transport Haloferax volcanii 

Abbreviations

CCCP

Carbonylcyanide m-chlorophenyl-hydrazone

DCCD

N,N′-dicyclohexylcarbodiimide

MES

2-[N-morpholino] ethane sulfonic acid

MOPS

3-[N-morpholino] propane sulfonic acid

TRIS

Tris (hydroxymethyl) aminomethane

TPP

tetraphenyl phosphonium

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References

  1. Bakker ER, Rottenberg H, Caplan SR (1976) An estimation of the light-induced electrochemical potential difference of protons across the membrane of Halobacterium halobium. Biochim Biophys Acta 440:557–572Google Scholar
  2. Epstein W, Whitelaw V, Hesse J (1978) A K+ transport ATPase in Escherichia coli. J Biol Chem 253:6666–6668Google Scholar
  3. Garty H, Caplan R (1977) Light-dependent rubidium transport in intact Halobacterium halobium cells. Biochim Biophys Acta 459:532–545Google Scholar
  4. Ginzburg M, Sachs L, Ginzburg B-Z (1971) Ion metabolism in Halobacterium halobium. J Membr Biol 5:78–101Google Scholar
  5. Juez G (1988) Taxonomy of extremely halophilic archaebacteria. In: Rodriguez-Valera F (ed) Halophilic bacteria, vol II. CRC, Boca Raton, pp 3–24Google Scholar
  6. Klein WL, Boyer PD (1972) Energization of active transport by Escherichia coli. J Biol Chem 247:7257–7265Google Scholar
  7. Konishi T, Murakami N (1984) Detection of two DCCD-binding components in the envelope membrane of Halobacterium halobium. FEBS Lett 169:283–286Google Scholar
  8. Konishi T, Murakami N (1988) ΔΨ-dependent gating of Na+/H+ exchange in Halobacterium halobium: a ΔμH+-driven Na+ pump. FEBS Lett 226:270–274Google Scholar
  9. Lanyi JK, Hilliker K (1976) Passive potassium ion permeability of Halobacterium halobium envelope membranes. Biochim Biophys Acta 448:181–184Google Scholar
  10. Lanyi JK, Silverman MP (1972) The state of binding of intracellular K+ in Halobacterium cutirubrum. Can J Microbiol 18:993–995Google Scholar
  11. Lanyi JK, Helgerson S, Silverman M (1979) Relationship between proton motive force and potassium ion transport in Halobacterium halobium vesicles envelope. Arch Biochem Biophys 193:329–339Google Scholar
  12. Meury J (1976) Transport du potassium chez Escherichia coli. Thesis. Univ. ParisGoogle Scholar
  13. Meury J, Lebail S, Kepes A (1980) Opening of potassium channels in Escherichia coli membranes by thiol reagents and recovery of potassium tightness. Eur J Biochem 113:33–38Google Scholar
  14. Meury J, Kepes A (1981) The regulation of potassium fluxes for the adjustment and maintenance of potassium levels in Escherichia coli. Eur J Biochem 19:165–170Google Scholar
  15. Mevarech M, Werczberger R (1985) Genetic transfer in Halobacterium volcanii. J Bacteriol 162:461–462Google Scholar
  16. Murakami N, Konishi T (1985) DCCD-sensitive, Na+-dependent H+-influx process coupled to membrane potential formation in membrane vesicles of Halobacterium halobium. J Biochem (Tokyo) 98:897–907Google Scholar
  17. Rhoads DB, Epstein W (1977) Energy coupling to net K+ transport in Escherichia coli K-12. J Biol Chem 253:1394–1401Google Scholar
  18. Richarme G (1985) Possible involvement of lipoic acid in binding protein-dependent transport systems in Escherichia coli. J Bacteriol 162:286–293Google Scholar
  19. Steward LMD, Bakker EP, Booth IR (1985) Energy coupling to K+ uptake via the Trk system in Escherichia coli: the role of ATP. J Gen Microbiol 131:77–85Google Scholar
  20. Tokuda H, Nakamura T, Unemoto T (1981) Potassium ion is required for the generation of pH-dependent membrane potential and ΔpH by the marine bacterium Vibrio alginolyticus. Biochemistry 20:4198–4203Google Scholar
  21. Wagner G, Hartmann R, Oesterhelt D (1978) Potassium uniport and ATP synthesis in Halobacterium halobium. Eur J Biochem 89:160–179Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Jean Meury
    • 1
  • Masamichi Kohiyama
    • 1
  1. 1.Biochimie Génétique, Institut Jacques MonodUniversité Paris VIIParis Cédex 05France

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