Summary
The expression of Ia-antigen on four different Epstein-Barr virus associated nasopharyngeal carcinomas xenografted into athymic mice could be detected by the monoclonal antibody OKIa. Xenografts of four additional head and neck tumors other than nasopharyngeal carcinoma and one xenograft of a metastatic melanoma cell line were negative for the Ia-antigen. Control antibodies OKT3, OKT4, OKT9, OKM1 and Leu7 were negative with all nasopharyngeal carcinomas and the non-nasopharyngeal carcinoma xenografts. Complement receptors as the presumed receptors for the Epstein-Barr virus could not be detected on xenografted nasopharyngeal carcinoma cells but were found on freshly prepared peripheral blood lymphocytes as well as on the Epstein-Barr virus transformed lymphoblastoid cell line QIMR-WIL. The possible role of the Ia-antigen on nasopharyngeal carcinoma cells in respect to the Epstein-Barr virus association of this malignancy is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bayliss GJ, Wolf H (1980) Epstein-Barr virus induced cell fusion. Nature 287:164–165
Daynes RA, Enam M, Krueger GG, Roberts LK (1983) Expression of Ia-antigen on epidermal keratinocytes after the grafting of normal skin to nude mice. J Immunol 130:1536–1539
Durum SK, Gershon RK (1982) Interleukin 1 can replace the requirement for Ia positive cells in the proliferation of antigen primed T cells. Proc Natl Acad Sci USA 79:4747–4752
Ebbers J, Lindenberger J, Meyer zum Gottesberge-Orsulakova A, Koldovsky P, Koldovsky U, Vosteen K-H (1985) Xenografting of nasopharyngeal carcinoma (NPC) into athymic mice. ORL (in press)
Fu S, Chiorazzi N, Wang CY, Montazeri G, Kunkel HG, Ko HS, Gottlieb AB (1978) Ia bearing T lymphocytes in man. Their identification and role in the generation of allogeneic helper activity. J Exp Med 148:1423–1428
Graessman A, Wolf H, Bornkamm GW (1980) Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA. Proc Natl Acad Sci USA 77:433–436
Halper J, Fu SM, Gottlieb AB, Winchester RJ, Kunkel HG (1979) Poor mixed lymphocyte reaction stimulatory capacity of leukemic B cells of chronic lymphocytic leukemia patients despite the presence of Ia antigens. J Clin Invest 64:1141–1148
Henle W, Henle G (1981) The association of Epstein-Barr virus with nasopharyngeal carcinoma. In: Grundmann E, Krueger GRF, Ablashi DV (eds) Nasopharyngeal carcinoma: cancer campaign 5. Fischer, Stuttgart New York, pp 1–6
Hudson L, Hay FC (1980) Practical immunology. Blackwell Scientific Publications, Oxford, pp 41–43
Jondal M, Klein G, Oldstone MBA, Bokish V, Yefenof E (1976) Surface markers on human B and T lymphocytes. VIII. Association between complement and Epstein-Barr virus receptors on human lymphoid cells. Scand J Immunol 5:401–410
Klein G (1973) The Epstein-Barr virus. In: Kaplan AS (ed) The herpesviruses. Academic Press, New York London, pp 521–555
Lalezari P (1984) Biological roles of tissue specific and systemic alloantigenes. In: Advances in immunobiology. Blood cell antigenes and bone marrow transplantation. Alan R Liss, New York, pp 55–75
Lampson LA, Levy R (1980) Two populations of Ia-like molecules on a human B cell line. J Immunol 125:293–299
Natali PG, DeMartino C, Quaranta V, Nicotra MR, Frezza F, Pellegrino MA, Ferrone S (1981) Expression of Ia-like antigens in normal human non lymphoid tissues. Transplantation 31:75–82
Robellino EM, Nachmann RL, Williams N, Winchester RJ, Ross GD (1979) Human megakaryocytes. I. Characterization of the membrane and cytoplasmic components of isolated marrow megakaryocytes. J Exp Med 149:1273–1276
Steel CM, Philipson J, Gardiner AE, Newton MS, McIntosh RV (1977) Possibility of EB virus preferentially transforming a subpopulation of human B lymphocytes. Nature 270:729–731
Stites DP (1976) Laboratory methods for detection of antigens and antibodies. In: Fudenberg HH, Stites DP, Caldwell JL, Wells JV (eds) Basic and clinical immunology. Lange Medical Publications. Los Altos, California
Trowbridge IS, Hyman A, Klein G (1977) Human B cell line deficient in the expression of B cell specific glycoproteins (GP 27,35). Eur J Immunol 7:640–645
Volsky DJ, Shapiro IM, Klein G (1980) Transfer of Epstein-Barr virus receptors to receptor-negative cells permits virus penetration and antigen expression. Proc Natl Acad Sci USA 77:5453–5457
Winchester RJ, Kunkel HG (1979) The human Ia system. Adv Immunol 28:221–292
Winchester RJ, Ross GD, Jarowski CI, Wang CY, Halper J, Broxmeyer HE (1977) Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation. Proc Natl Acad Sci USA 74:4012–4015
Winchester RJ, Meyers PA, Broxmeyer HE, Wang CY, Moore MAS, Kunkel HG (1978) Inhibition of human erythropoetic colony formation in culture by treatment with Ia antisera. J Exp Med 148:613–615
Wolf H, Bayliss GJ, Wilmes E (1981) Biological properties of Epstein-Barr virus. In: Grundmann E, Krueger GRF, Ablashi DV (eds) Nasopharyngeal carcinoma: cancer campaign 5. Fischer, Stuttgart New York, pp 101–110
Wolf H, Haus M, Wilmes E (1984) Persistence of Epstein-Barr virus in the parotid gland. J Virol 51:795–798
Author information
Authors and Affiliations
Additional information
This work was supported by the Deutsche Forschungsgemeinschaft
Rights and permissions
About this article
Cite this article
Ebbers, J., Koldovsky, P. & Vosteen, K.H. The expression of Ia-antigen on nasopharyngeal carcinomas xenografted into nude mice. Arch Otorhinolaryngol 242, 209–215 (1985). https://doi.org/10.1007/BF00454423
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00454423