Applied Microbiology and Biotechnology

, Volume 19, Issue 6, pp 430–434 | Cite as

Isolation and characterization of a symbiotic cellulolytic mixed bacterial culture

  • Marya de la Torre
  • Carlos Casas Campillo
Short Communication


By using batch-culture enrichment techniques a mixed culture of two bacterial spe cies identified as Cellulomonas flavigena and Xanthomonas sp was isolated. The capacity of both bacteria to grow as pure cultures in a min eral medium with alkaline pretreated sugar cane bagasse or cellobiose was tested. C. flavigena as pure culture was able to grow on both substrates only when yeast extract or biotin and thiamine were added to the culture medium, while Xanthomonas sp. could not grow on sugar cane ba gasse, but assimilated cellobiose if yeast extract was supplied. However, both bacteria in mixed culture grew very well on both substrates and did not require any growth factor. It was concluded that the interaction was favourable to both species. The mixed culture had the capacity to degrade a number of different agricul tural wastes and to use them as the sole carbon and energy source for the production mainly of biomass. More than 80% of pineapple bagasse, without chemical pretreatment, was used up by the microbial system.


Sugar Cane Thiamine Cellobiose Pure Culture Bagasse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Marya de la Torre
    • 1
  • Carlos Casas Campillo
    • 1
  1. 1.Department of Biotechnologie and BioengineeringCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMéxicoMexico

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