Spirulina culture in sea-water
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Laboratory experiments using small raceway ponds have shown that Spirulina maxima can be adapted easily to grow in sea-water supplemented with nitrate, phosphate, bicarbonate, and Fe-EDTA. To prevent precipitate formation, phosphate was supplied by diffusion through a dialysis membrane; the amount of Na-bicarbonate added was low (100 ppm) and the pH was kept in the range 8.6–8.8 by bubbling CO2 into the culture.
No significant differences have been noticed in productivity or in the chemical composition of the biomass between cultures in sea-water and in the standard bicarbonate medium. Cultures subjected to light/dark cycles of 12/12 h showed a higher respiration rate in sea-water than in the bicarbonate medium.
The higher weight loss in the sea-water medium in the dark was counterbalanced by an increased synthesis of carbohydrates during the light period.
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