Applied Microbiology and Biotechnology

, Volume 19, Issue 6, pp 384–386 | Cite as

Spirulina culture in sea-water

  • Riccardo Materassi
  • Mario Tredici
  • Waldemaro Balloni
Biotechnology

Summary

Laboratory experiments using small raceway ponds have shown that Spirulina maxima can be adapted easily to grow in sea-water supplemented with nitrate, phosphate, bicarbonate, and Fe-EDTA. To prevent precipitate formation, phosphate was supplied by diffusion through a dialysis membrane; the amount of Na-bicarbonate added was low (100 ppm) and the pH was kept in the range 8.6–8.8 by bubbling CO2 into the culture.

No significant differences have been noticed in productivity or in the chemical composition of the biomass between cultures in sea-water and in the standard bicarbonate medium. Cultures subjected to light/dark cycles of 12/12 h showed a higher respiration rate in sea-water than in the bicarbonate medium.

The higher weight loss in the sea-water medium in the dark was counterbalanced by an increased synthesis of carbohydrates during the light period.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ajala Jimenez FA, Benavente RB (1982) An improved cheap culture medium for the blue-green microalgae Spirulina. Eur J Appl Microbiol Biotechnol 15: 198–199Google Scholar
  2. Faucher O, Coupal B, Leduy A (1979) Utilization of seawater-urea as a culture medium for Spirulina maxima. Can J Microbiol 25: 752–759Google Scholar
  3. Healey FP (1973) Characteristic of phosphorus deficiency in Anabaena. J Phycol 9: 383–394Google Scholar
  4. Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5B. Academic Press, London New York, pp 209–344Google Scholar
  5. Koroleff F (1976) Determination of nutrients. In: Grasshoff K (ed) Methods of seawater analysis. Verlag Chemie, Weinheim New York, pp 117–181Google Scholar
  6. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275Google Scholar
  7. Murphy J, Riley JP (1958) A single solution method for the determination of soluble phosphate in sea-water. J Mar Biol Assoc UK 37: 9–14Google Scholar
  8. Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27: 31–36Google Scholar
  9. Paoletti C, Pushparaj B, Tomaselli-Feroci L (1975) Ricerche sulla nutrizione minerale di Spirulina platensis. Atti XVII Congr Naz Microbiol 2: 833–839Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Riccardo Materassi
    • 1
    • 2
  • Mario Tredici
    • 1
    • 2
  • Waldemaro Balloni
    • 1
    • 2
  1. 1.Centro di Studio dei Microorganismi Autotrofi del C.N.R.Università di FirenzeFirenzeItaly
  2. 2.Istituto di Microbiologia agraria e tecnicaUniversità di FirenzeFirenzeItaly

Personalised recommendations