Zusammenfassung
Die Beziehungen zwischen Parotin (Speicheldrüsenhormon) und der menschlichen Parotis wurden mit der indirekten Technik der Enzym-Antikörper-Methode untersucht. Die Ergebnisse zeigen, daß Parotin selektiv an die Zellen des Gangsystems der menschlichen Parotis gebunden ist.
Summary
The relationship between parotin and human parotid gland was investigated using indirect technique of enzyme antibody method. Normal rabbit IgG has non-specific affinity to duct cells of human parotid gland, but it was suppressed by adding bovine serum albumin. Anti-parotin rabbit IgG has no reaction products on the sections of adult human parotid gland. When the sections, initially incubated with parotin solution in PBS, were treated with anti-parotin rabbit IgG containing bovine serum albumin, and then HRPO-labeled anti-rabbit gamma globulin antibodies (swine IgG), intercalated and striated ducts excepting excretory ducts were stained with diaminobenzidine reaction, but also acinar cells did not. These results indicate that parotin is bound specifically on duct cells of human parotid gland.
This is a preview of subscription content, log in via an institution to check access.
References
Aonuma S, Kohama Y, Nakajin S, Egawa H (1974) The study of physiological chemistry on a subunit of salivary gland hormone. Folia Endocrinol Jpn 50: 1468–1476
Aonuma S, Kohama Y, Nakajin S, Yashiki S, Egawa H, Wada M, Komiyama Y (1976) The study of physiological chemistry on a subunit of salivary gland hormone (2). Folia Endocrinol Jpn 52: 93–104
Ito Y (1960) Parotin: a salivary gland hormone. Ann NY Acad Sci 85: 228–310
Fahey JL (1976) Chromatographic separation of immunoglobulin. In: Williams CA, Chase NW (eds) Method in immunology and immunochemistry, vol 1. Academic Press, New York London, pp 321–332
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14: 291–302
Kirikae I (1974) Sjögren's disease or syndrome. In: Kirikae I (ed) Modern Oto-Rhino-Laryngology. Nanzando Company, Tokyo, p 452
Kitamura T (1971) Function disturbance — xerostomia. In: Goto T, Shiraiwa T, Kitamura T (eds) Oto-Rhino-Laryngology, vol 2. Igakushoin Ltd, Tokyo, p 1341
Ogata T (1955) The internal secretion of salivary gland. Endocrinol Jpn 2: 247–261
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Iizuka, K., Togawa, K., Konno, A. et al. The specific binding of parotin on duct cells of human parotid gland. Arch Otorhinolaryngol 229, 143–148 (1980). https://doi.org/10.1007/BF00454237
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00454237