Archives of oto-rhino-laryngology

, Volume 236, Issue 3, pp 281–293 | Cite as

Respiration rate in human pituitary tumor explants

  • Matti Anniko
  • Dan Bagger-Sjöbäck
  • Ragnar Hultborn


Studies on the respiration rate of human pituitary tumor tissue have so far been lacking in the literature. This study presents the results from four adenomas causing acromegaly, all with different clinical degrees of the disease. Determination of oxygen uptake was performed in vitro with a spectrophotorespirometric system. Pieces of the tumors were explanted to an organ culture system with a high degree of stability. The secretion rate of growth hormone (GH) and prolactin (PRL) was determined.

After 4–8 days in vitro, specimens were analyzed for respiration rate. This was approximately 1–1.5 μl O2/h/ μg dry weight. The activity of the pituitary tumor tissue was characterized by both the hormone secretion rate and the respiration rate. Particularly active foci were found to occur in the adenoma tissue. Depending on the individual tumor, the GH secretion rate was approximately 0.1–100 pmol/μg dry weight/h and PRL secretion rate approximately 0.4–18 μg/μg dry weight/h. The respiration rate — as is also the hormone secretion rate — is dependent on the time in vitro prior to analysis. The respiration rate in individual tumors is a parameter which does not reflect GH or PRL serum levels or clinical activity of the disease.

Key words

Human pituitary tumor in vitro Respiration GH PRL 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Matti Anniko
    • 1
  • Dan Bagger-Sjöbäck
    • 1
  • Ragnar Hultborn
    • 2
  1. 1.Department of OtolaryngologyKarolinska Hospital, and King Gustav V Research Institute, Karolinska InstituteStockholmSweden
  2. 2.Departments of Histology and Clinical OncologyUniversity of Göteborg, Jubileumskliniken, Sahlgrenska HospitalGöteborgSweden

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