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Archives of oto-rhino-laryngology

, Volume 236, Issue 3, pp 281–293 | Cite as

Respiration rate in human pituitary tumor explants

  • Matti Anniko
  • Dan Bagger-Sjöbäck
  • Ragnar Hultborn
Article
  • 17 Downloads

Summary

Studies on the respiration rate of human pituitary tumor tissue have so far been lacking in the literature. This study presents the results from four adenomas causing acromegaly, all with different clinical degrees of the disease. Determination of oxygen uptake was performed in vitro with a spectrophotorespirometric system. Pieces of the tumors were explanted to an organ culture system with a high degree of stability. The secretion rate of growth hormone (GH) and prolactin (PRL) was determined.

After 4–8 days in vitro, specimens were analyzed for respiration rate. This was approximately 1–1.5 μl O2/h/ μg dry weight. The activity of the pituitary tumor tissue was characterized by both the hormone secretion rate and the respiration rate. Particularly active foci were found to occur in the adenoma tissue. Depending on the individual tumor, the GH secretion rate was approximately 0.1–100 pmol/μg dry weight/h and PRL secretion rate approximately 0.4–18 μg/μg dry weight/h. The respiration rate — as is also the hormone secretion rate — is dependent on the time in vitro prior to analysis. The respiration rate in individual tumors is a parameter which does not reflect GH or PRL serum levels or clinical activity of the disease.

Key words

Human pituitary tumor in vitro Respiration GH PRL 

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References

  1. 1.
    Anniko M (1981) Die Organ-Kultur als Modell zur Untersuchung menschlicher Hypophysentumoren. Laryngol Rhinol Otol 60: 304–307Google Scholar
  2. 2.
    Anniko M (1982) Das Verhalten des menschlichen Hypophysentumors in vitro. Eine Analyse der Tumorbiologie von 51 konsekutiv operierten Fällen. Laryngol Rhinol Otol (im Druck)Google Scholar
  3. 3.
    Anniko M, Eneroth P, Werner S, Wersäll J (1979) In vitro preservation of human pituitary tumours in organotypic differentiation. Acta Otolaryngol (Stockh) 88: 424–443Google Scholar
  4. 4.
    Anniko M, Wersäll J (1980) Human pituitary tumours in organ culture with correlation to clinical data. Acta Otolaryngol (Stockh) 89: 249–257Google Scholar
  5. 5.
    Anniko M, Holm L-E, Silferswärd C, Tribukait B, Werner S, Wersäll J (1981) DNA characteristics of human pituitary tumours. Acta Otolaryngol [Suppl] (Stockh) 379: 1–28Google Scholar
  6. 6.
    Anniko M, Werner S, Wersäll J (1980) Growth hormone and prolactin production in human pituitary tumours in organ culture. Acta Otolaryngol (Stockh) 90: 143–151Google Scholar
  7. 7.
    Cardell RR, Knighton RS (1966) The cytology of a human pituitary tumour. An electron microscopic study. Trans Microsc Soc 85: 58–78Google Scholar
  8. 8.
    Herlitz H, Hultborn R (1974) A microspectrophotometric technique for determination of respiration of comparison to the cartesian diver method. Acta Physiol Scand 90: 594–601Google Scholar
  9. 9.
    Hultborn R (1974) A spectrophotometric method for analysis of oxygen consumption in vivo on the microscale. Acta Physiol Scand Suppl 404: 1–47Google Scholar
  10. 10.
    Hymer WC, Snyder J, Wilfinger W, Bergland R, Fischer B, Pearson O (1976) Characterization of mammotrophs separated from the human pituitary gland. J Natl Cancer Inst 57: 995–1007Google Scholar
  11. 11.
    Kohler PO, Bridson WE, Rayford PL, Kohler SE (1969) Hormone production by human pituitary adenomas in culture. Metabolism 18: 783–788Google Scholar
  12. 12.
    Lucas DRA (1965) A respirometer for organ cultures. Biochem J 97: 769–775Google Scholar
  13. 13.
    Lipson CG, Beitins IZ, Kornblith PD, McArthur JW, Friesen HG, Kliman B, Kjellberg RN (1978) Tissue culture studies on human pituitary tumours: radioimmunoassayable anterior pituitary hormones in the culture medium. Acta Endocrinol (Copenh) 88: 239–249Google Scholar
  14. 14.
    Martin-Comin J, Robyn C (1976) Comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries. J Histochem Cytochem 24: 1012–1016Google Scholar
  15. 15.
    Pasteels JL, Gausset P, Danguy A, Ectors F (1972) Immunofluorescent studies on prolactin and the pituitary. In: Boyns AR, Griffith K (eds) Prolactin and carcinogenesis. Alpha Omega, Cardiff, pp 128–136Google Scholar
  16. 16.
    Peillon F, Gourmelen M, Donnadieu M, Brandi A, Sevaux D, Pham Huu Trung MT (1975) Organ culture of human somatotrophic pituitary adenomas: ultrastructure and growth hormone production. Acta Endocrinol (Copenh) 79: 217–229Google Scholar
  17. 17.
    Peillon F, Cesselin F, Garnier PE, Brandi AM, Donnadieu M, L'Hermite M, Dubois MP (1978) Prolactin secretion and synthesis in short and long-term organ culture of pituitary tumours from acromegalic patients. Acta Endocrinol (Copenh) 87: 701–715Google Scholar
  18. 18.
    Peillon F, Cesselin F, Bression D, Zygelman N, Brandi AM, Nousbaum A, Mauborgne A (1979) In vitro effect of amine and L-dopa on prolactin and growth hormone release from human pituitary adenomas. J Clin Endocrinol Metab 49: 737–741Google Scholar
  19. 19.
    Skyler JW, Rogol AD, Lovenberg N, Knazek RA (1977) Characterization of growth hormone and prolactin produced by human pituitary in culture. Endocrinology 100: 283–291Google Scholar
  20. 20.
    Snyder J, Hymer WC, Wilfinger WW (1978) Culture of human pituitary prolactin and growth hormone cells. Cell Tissue Res 191: 379–388Google Scholar
  21. 21.
    Zimmerman EA, Defendini R, Franz AG (1974) Prolactin and growth hormone in patients with pituitary adenomas. A correlative study of hormone in tumour and plasma by immunoperoxidase technique and radioimmunoassay. J Clin Endocrinol Metab 38: 577–585Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Matti Anniko
    • 1
  • Dan Bagger-Sjöbäck
    • 1
  • Ragnar Hultborn
    • 2
  1. 1.Department of OtolaryngologyKarolinska Hospital, and King Gustav V Research Institute, Karolinska InstituteStockholmSweden
  2. 2.Departments of Histology and Clinical OncologyUniversity of Göteborg, Jubileumskliniken, Sahlgrenska HospitalGöteborgSweden

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