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A whole-organ serial-sectioning technique for histologic examination of laryngeal specimens

Eine Organserienschnittechnik zur histologischen Untersuchung von Kehlkopfpräparaten

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Zusammenfassung

Zur Aufarbeitung von Kehlkopfpräparaten wird eine schonende histologische Technik mit Paraffineinbettung angegeben. Die Entkalkung und Entwässerung werden im Vakuum ohne Elektrolyse oder Ultraschall durchgeführt. Die Schnittdicke der einzelnen histologischen Präparate beträgt 5 μm. Die Aufarbeitung der Kehlköpfe dauert je nach Größe 3–4 1/2 Wochen. Auf die Bedeutung dieser Technik für die klinische Diagnostik und die Kontrolle der Computer-Tomographie des Kehlkopfes wird hingewiesen.

Summary

A preserving histologic technique with paraffin-embedding for the processing of laryngeal specimens is described. Decalcification and dehydration are performed in vacuum without electrolytic or ultrasonic treatment. The thickness of the individual histologic sections is 5 μm. Processing of the larynx takes 3–4 1/2 weeks, depending on size. The significance of this technique in the histologic control of clinical diagnosis and/or high-resolution computerized tomography is pointed out.

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Meyer-Breiting, E., Weith, E. A whole-organ serial-sectioning technique for histologic examination of laryngeal specimens. Arch Otorhinolaryngol 237, 7–15 (1982). https://doi.org/10.1007/BF00453711

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  • DOI: https://doi.org/10.1007/BF00453711

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