Archives of oto-rhino-laryngology

, Volume 244, Issue 1, pp 66–68 | Cite as

Immunohistochemical demonstration of the co-expression of vimentin and cytokeratin(s) in the guinea pig cochlea

  • M. Kasper
  • P. Stosiek
  • A. Varga
  • U. Karsten
Article

Summary

Using a decalcification procedure for guinea pig petrous bones, we were able to prepare cryostat sections for immunohistochemical studies. A panel of monoclonal antibodies against intermediate filament proteins was then used to show a co-expression of cytokeratin and vimentin in the epithelial cells of the stria vascularis.

Key words

Cytokeratin Vimentin Guinea pig cochlea Stria vascularis 

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References

  1. 1.
    Altmannsberger M, Osborn M, Schauer A, Weber K (1981) Antibodies to different intermediate filament proteins. Lab Invest 45:427–434Google Scholar
  2. 2.
    Anniko M, Thornell L-E, Gustavsson H, Virtanen I (1986) Intermediate filaments in the newborn inner ear of the mouse. ORL 48:98–106Google Scholar
  3. 3.
    Holthöfer H, Miettinen A, Lehto V-P, Lehtonen E, Virtanen I (1984) Expression of vimentin and cytokeratin types of intermediate filament proteins in developing and adult human kidney. Lab Invest 50:552–559Google Scholar
  4. 4.
    Jonsson R, Tarkowski A, Klareskog L (1986) A demineralization procedure for immunohistopathological use. EDTA treatment preserves lymphoid cell surface antigens. J Immunol Methods 88:109–114Google Scholar
  5. 5.
    Karsten U, Widmaier R, Kunde D (1983) Monoclonal antibodies against antigens of the human mammary carcinoma cell line MCF-7. Arch Geschwulstforsch 53:513–519Google Scholar
  6. 6.
    Kasper M, Karsten U, Stosiek P (1986) Detection of cytokeratin(s) in epithelium of human plexus choroideus by monoclonal antibodies. Acta Histochem 78:101–103Google Scholar
  7. 7.
    Kasper M, Goertchen R, Stosiek P, Perry G, Karsten U (1986) Coexistence of cytokeratin, vimentin and neurofilament protein in human choroid plexus. An immunohistochemical study of intermediate filaments in neuroepithelial tissues. Virchows Arch [A] 410:173–177Google Scholar
  8. 8.
    Kasper M, Stosiek P, Typlt H, Karsten U (1987) Histological evaluation of three new monoclonal anti cytokeratin antibodies. 1. Normal tissues. Eur J Cancer Clin Oncol (in press)Google Scholar
  9. 9.
    Kasper M, Münchow T, Stosiek P, Karsten U (1987) Similarities in the composition of intermediate filaments between human ciliary and choroid plexus epithelia —correlation with similar secretory functions. Acta Histochem (in press)Google Scholar
  10. 10.
    Lane EB, Hogan BLM, Kurkinen M, Garrels JI (1983) Coexpression of vimentin and cytokeratins in parietal endoderm cells of early mouse embryo. Nature 303:701–704Google Scholar
  11. 11.
    La Rocca PJ, Rheinwald JG (1984) Coexpression of simple epithelial keratins and vimentin by human mesothelium and mesotheliom as in vivo and in culture. Cancer Res 44:2991–2999Google Scholar
  12. 12.
    Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24Google Scholar
  13. 13.
    Nakane PK (1968) Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem 16:557–560Google Scholar
  14. 14.
    Osborn M, Weber K (1983) Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48:372–394Google Scholar
  15. 15.
    Regauer S, Franke WW, Virtonen I (1985) Intermediate filament cytoskeleton of amnion epithelium and cultured amnion epithelial cells: experession of epidermal cytokeratins in cells of a simple epithelium. J Cell Biol 100:997–1009Google Scholar
  16. 16.
    Watanabe K, Ogawa A (1984) Carboanhydraseaktivität in der Stria vascularis und in den dunklen Zellen des vestibulären Labyrinths. Ann Otol 93:262–266Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. Kasper
    • 1
  • P. Stosiek
    • 1
  • A. Varga
    • 1
  • U. Karsten
    • 2
  1. 1.Pathological Institute, District HospitalGörlitzGerman Democratic Republic
  2. 2.Department of ImmunologyInstitute of Molecular Biology, Academy of Sciences of the GDRBerlin-BuchGerman Demokratic Republic

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