Abstract
Axenic culture of photosynthetic purple sulphur bacterium, isolated from a paper mill effluent, grew best with ammonium chloride as nitrogen source. Cells grown with N2 or ammonium chloride produced hydrogen with initial lag periods of 24 and 48 h, respectively. The maximum amount of hydrogen was evolved by cells grown with N2 and with malate as electron donor.
This is a preview of subscription content, log in via an institution to check access.
References
Gest H. 1972 Energy conversion and generation of reducing power in bacterial photosynthesis. Advances in Microbial Physiology 7, 243–282.
Gest H. & Kamen M.D. 1949 Photoproduction ofmolecular hydrogen by Rhodospirillum rubrum. Science 109, 558–559.
Hillmer P. & Gest H. 1977 H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: H2 production by growing cultures. Journal of Bacteriology 129, 724–731.
Ohta Y. & Mitsui A. 1981 Enhancement of hydrogen production by marine Chromatium sp. Miami PBS 1071 grown in molecular nitrogen. Advances in Biotechnology 2, 303–307.
Renuka B.R., Rajani B. & Vinaya Kumar M. 1987 Isolation and characterization of photosynthetic bacteria from Industrial effluents. Proceedings of Indian National Science Academy B 53, 69–72.
Sweet J.W. & Burris R.H. 1981 Inhibition of nitrogenase activity by NH sup+inf4 in Rhodospirillum rubrum. Journal of Bacteriology 145, 824–831.
Van Niel C. B. 1971 Techniques for the enrichment, isolation and maintenance of photosynthetic bacteria. Methods in Enzymology 23, 3–28.
Vincenzini M., Materassi R., Tredici M.R. & Florenzano G. 1982 Hydrogen production by immobilized cells. 1. Light dependent dissimilation of organic substances by Rhodopseudomonas palustris. International Journal of Hydrogen Energy 7, 231–236.
Additional information
R. Lakshmi is with the Microbial Physiology Laboratory, Department of Botany, and H. Polasa is with the Department of Microbiology, Osmania University, Hyderabad 500 007, India.
Rights and permissions
About this article
Cite this article
Lakshmi, R., Polasa, H. Influence of nitrogen source on hydrogen generation by a photosynthetic bacterium. World J Microbiol Biotechnol 7, 619–621 (1991). https://doi.org/10.1007/BF00452844
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00452844