Summary
A low-affinity and a high-affinity sylose proton symport operated simultaneously in both starved and non-starved cells of Pichia stipitis. Glucose competed with xylose for transport by the low-affinity system and inhibited xylose transport by the high-affinity system non-competitively. The low affinity system was subject to substrate inhibition when glucose but not when xylose was the substrate. The differences between the characteristics of monosaccharide transport by Pichia stipitis and its imperfect state, Candida shehatae, are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alcorn ME, Griffin CC (1978) A kinetic analysis of D-xylose transport in Rhodotorula glutinis. Biochim Biophys Acta 510:361–371
Du Preez JC, Prior BA (1985) A quantitative screening of some xylose-fermenting yeast isolates. Biotechnol Lett 7:241–246
Du Preez JC, Bosch M, Prior BA (1986) The fermentation of hexose and pentose sugars by Candida shehatae and Pichia stipitis. Appl Microbiol Biotechnol 23:228–233
Görts CPM (1969) Effect of glucose on the activity and the kinetics of the maltose-uptake system and of α-glucosidase in Saccharomyces cerevisiae. Biochim Biophys Acta 184:299–305
Höfer M, Misra P (1978) Evidence for a proton symport in the yeast Rhodotorula gracilis (glutinis). Biochem J 172:15–22
Kreger-van Rij NJW (1970) Pichia Hansen. In: Lodder J (ed) The Yeasts, 2nd edition, North-Holland Publishing Company, Amsterdam, p 533
Lucas C, van Uden N (1986) Transport of hemicellulose monomers in the xylose-fermenting yeast Candida shehatae. Appl Microbiol Biotechnol 23:491–495
Matern H, Holzer H (1977) Catabolite inactivation of the galactose uptake system in yeast. J Biol Chem 252:6399–6402
Meyer SA, Ahearn DG, Yarrow D (1984) Candida Berkhout. In: Kreger-van Rij NJW (ed) The Yeasts, 3rd edition, Elsevier Science Publishers, Amsterdam, p 794
Okada H, Halvorson HO (1964a) Uptake of α-thioethyl D-glucopyranoside by Saccharomyces cerevisiae. I. The genetic control of facilitated diffusion and active transport. Biochim Biophys Acta 82:538–546
Okada H, Halvorson HO (1964b) Uptake of α-thioethyl D-glucopyranoside by Saccharomyces cerevisiae. II. General characteristics of an active transport system. Biochim Biophys Acta 82:547–555
Spencer-Martins I, van Uden N (1985) Catabolite interconversion of glucose transport systems in the yeast Candida wickerhamii. Biochim Biophys Acta 812:168–172
Van Uden N (1967) Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Arch Microbiol 58:155–168
Van Uden N, Buckley H (1970) Candida Berkhout. In: Lodder J (ed) The Yeasts, 2nd edition, North-Holland Publishing Company, Amsterdam, p 1046
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kilian, S.G., van Uden, N. Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis . Appl Microbiol Biotechnol 27, 545–548 (1988). https://doi.org/10.1007/BF00451629
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00451629