Marine Biology

, Volume 95, Issue 1, pp 103–113 | Cite as

Importance of food quality in determining development and survival of Calanus pacificus (Copepoda: Calanoida)

  • M. E. Huntley
  • P. Ciminiello
  • M. D. G. Lopez
Article

Abstract

Mixed zooplankton were collected in June and July of 1985 and 1986 from La Jolla Bay, California, USA, and experiments were conducted to determine how selected dinoflagellates affect development and survival of nauplius larvae of Calanus pacificus. We raised nauplii from eggs on nine species of dinoflagellates at concentrations generally >300 μg C l-1, and compared their development and survival to controls reared using the diatom Thalassiosira weissflogii or filtered seawater. Experiments were conducted for 6 d at 17°C. Development and survival rates of the nauplii fell clearly into one of two groups, depending upon the phytoplankton used as food. The first group was characterized by high development rate (0.46 to 0.84 stage d-1), and by >27% of the original cohort surviving to at least Nauplius IV or V. The five species producing this result were Gymnodinium simplex, G. splendens, Exuviaella marie-lebourae, Gyrodinium dorsum, and T. weissflogii. The second group was characterized by a development rate similar to that in filtered seawater (0.21 to 0.34 stage d-1), and by nauplii generally failing to molt past the first feeding stage (Nauplius III), often accompanied by high mortality. The five species producing this result were Gyrodinium resplendens, Ptychodiscus brevis, Glenodinium sp., Amphidinium carterae, and Gonyaulax grindleyi. Development rate and survival were not related to cell size or cell carbon, nor to shape or texture (thecate vs athecate dinoflagellates). Poor growth could be related to the absence of some important, but unidentified, nutritional factors. Alternatively, it could be caused by the presence of plant secondary metabolites which are deleterious to growth, a factor we suspect in P. brevis in particular. Prefeeding nauplii exposed to P. brevis lost neuromuscular control prior to becoming lethargic and dying; nutritional deficiencies may not explain these effects. Methods employed in this study provide useful bioassays for detecting chemical interactions between marine plants and animals. Lethal or sublethal effects of dinoflagellates on their most likely potential predators — copepods — may partially explain why they form significant blooms.

Keywords

Phytoplankton Development Rate Dinoflagellate Nutritional Deficiency Poor Growth 

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Literature cited

  1. Anderson, R. J., M. J. LeBlanc and F. W. Sum: 1-(2,6,6-trimethyl-4-hydroxy-cyclohexenyl)-1,3-butanediene, an extracellular metabolite from the dinoflagellate Prorocentrum minimum. J. org. Chem. 45, 1169–1190 (1980)Google Scholar
  2. Baden, D. G.: Marine food-borne dinoflagellate toxins. Int. Rev. Cytol. 82, 99–150 (1968)Google Scholar
  3. Baden, D. G., T. Mende, W. Lichter and L. Wellham: Crystallization and toxicology of T34: a major toxin from Florida's red tide organism (Ptychodiscus brevis). Toxicon 19, 455–462 (1981)Google Scholar
  4. Beers, J. R., F. M. H. Reid, and G. L. Stewart: Microplankton population structure in southern California nearshore waters during late spring. Mar. Biol. 60, 209–226 (1980)Google Scholar
  5. Corkett, C. and I. A. McLaren: Relationship between development rate of eggs and older stages of copepods. J. mar. biol. Ass. U.K. 50, 161–168 (1970)Google Scholar
  6. Eppley, R. W., R. W. Holmes and J. D. H. Strickland: Sinking rates of marine phytoplankton measured with a fluorometer. J. exp. mar. Biol. Ecol. 1, 191–208 (1967)Google Scholar
  7. Fernández, F.: Nutrition studies in the nauplius larva of Calanus pacificus (Copepoda: Calanoida). Mar. Biol. 53, 131–147 (1979a)Google Scholar
  8. Fernández, F.: Particle selection in the nauplius of Calanus pacificus. J. Plankton Res. 1, 313–328 (1979b)Google Scholar
  9. Fix Wichmann, C., W. P. Niemczura and H. K. Shnoes: Structures of two novel toxins from Protogonyaulax. J. Am. chem. Soc. 103, 6977–6978, (1981)Google Scholar
  10. Hitchcock, G. L.: A comparative study of the size-dependent organic composition of marine diatoms and dinoflagellates. J. Plankton Res. 4, 363–378 (1982)Google Scholar
  11. Huntley, M. E.: Yellow water in La Jolla Bay, California, July 1980. II. Suppression of zooplankton grazing. J. exp. mar. Biol. Ecol. 63, 81–91 (1982)Google Scholar
  12. Huntley, M. E.: A method for estimating food-limitation and potential production of zooplankton communities. Arch. Hydrobiol. (Beih. Ergebn. Limnol.) 21, 41–55 (1985)Google Scholar
  13. Huntley, M. E., K.-G. Barthel, and J. L. Star: Particle rejection by Calanus pacificus: discrimination between similarly sized particles. Mar. Biol. 74, 151–160 (1983)Google Scholar
  14. Huntley, M. (E.) and E. R. Brooks: Effects of age and food availability on diel vertical migration of Calanus pacificus. Mar. Biol. 71, 23–31, (1982)Google Scholar
  15. Huntley, M. E., P. Sykes, S. Rohan and V. Marin: Chemicallymediated rejection of dinoflagellate prey by the copepods Calanus pacificus and Paracalanus parvus: mechanism, occurrence and significance. Mar. Ecol. Prog. Ser. 28, 105–120 (1986)Google Scholar
  16. Ives, J. D.: The relationship between Gonyaulax tamarensis cell toxin levels and copepod ingestion rates. In: Toxic dinoflagellates, pp 413–418. Ed. by D. M. Anderson, A. M. White and D. Baden. New York: Elsevier Science 1985Google Scholar
  17. Landry, M. R.: Population dynamics and production of a planktonic marine copepod, Acartia clausii, in a small temperate lagoon on San Juan Island, Washington. Int. Revue ges. Hydrobiol. 63, 77–119 (1978)Google Scholar
  18. Lin, Y.-Y., M. Risk, S. M. Ray, D. Van Engen, J. Clardy, J. Golik, J. C. James, and K. Nakanishi: Isolation and structure of brevetoxin B from the “red tide” dinoflagellate Ptychodiscus brevis (Gymnodinium breve). J. Am. chem. Soc. 103, 6773–6775 (1981)Google Scholar
  19. Loeblich, III, A. R.: A seawater medium for dinoflagellates and the nutrition of Cachonina niei. J. Phycol. 11, 80–86 (1975)Google Scholar
  20. Lund, J. W., C. Kipling and E. D. LeCren: The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting. Hydrobiologia 11, 143–170 (1958)Google Scholar
  21. Marshall, S. M. and A. P. Orr: The biology of a marine copepod, 195 pp. Edinburgh: Oliver & Boyd 1955Google Scholar
  22. Marshall, S. M. and A. P. Orr: On the biology of Calanus finmarchicus. IX. Feeding and digestion in the young stages. J. mar. biol. Ass. U.K. 35, 587–603 (1956)Google Scholar
  23. McLaren, I. A.: Generation lengths of some temperate marine copepods: estimation, prediction and implications. J. Fish. Res. Bd Can. 35, 1330–1342 (1978)Google Scholar
  24. Mullin, M. and E. R. Brooks: Laboratory culture, growth rate and feeding behavior of a planktonic marine copepod. Limnol. Oceanogr. 12, 657–666 (1967)Google Scholar
  25. Mullin, M. and E. R. Brooks: Growth and metabolism of two planktonic, marine copepods as influenced by temperature and type of food. In: Marine food chains, pp 74–95. Ed. by J. Steele. Edinburgh: Oliver & Boyd 1970aGoogle Scholar
  26. Mullin, M. and E. R. Brooks: The effect of concentration of food on body weight, cumulative ingestion and rate of growth of the marine copepod, Calanus helgolandicus. Limnol. Oceanogr. 15, 748–755 (1970b)Google Scholar
  27. Mullin, M., P. R., Sloan and R. W. Eppley: Relationship between carbon content, cell volume and area in phytoplankton. Limnol. Oceanogr. 11, 307–311 (1966)Google Scholar
  28. Nassogne, A.: Influence of food organisms on the development and culture of pelagic copepods. Helgoländer wiss. Meeresunters. 20, 333–345 (1970)Google Scholar
  29. Omori, M. and T. Ikeda: Methods in marine zooplankton ecology, 332 pp. New York: John Wiley & Sons 1984Google Scholar
  30. Paffenhöfer, G.-A.: Cultivation of Calanus helgolandicus under controlled conditions. Helgoländer wiss. Meeresunters. 20, 346–359 (1970)Google Scholar
  31. Paffenhöfer, G.-A.: Grazing and ingestion rates of nauplii, copepodids and adults of the marine planktonic copepod Calanus helgolandicus. Mar. Biol. 11, 286–298 (1971)Google Scholar
  32. Reeve, M. R.: Large cod-end revervoirs as an aid to the live collection of zooplankton. Limnol. Oceanogr. 26, 577–579 (1981)Google Scholar
  33. Rhoades, D.: Evolution of plant chemical defeses against herbivores. In: Herbivores: their interaction with secondary plant metabolites, pp 3–54. Ed. by G. A. Rosenthal and D. H. Janzen. New York: Academic Press 1979Google Scholar
  34. Riley, G. A.: Factors controlling phytoplankton populations on Georges Bank. J. mar. Res. 6, 54–73 (1946a)Google Scholar
  35. Riley, G. A.: A theoretical analysis of the zooplankton population of Georges Bank. J. mar. Res. 6, 104–113 (1946b)Google Scholar
  36. Risk, M., Y. Y. Lin, R. D. McFarlane, V. Ramunujam, L. I. Smith and N. M. Trieff: Purification and chemical studies on a major toxin from Gymnodinium breve. In: Toxic dinoflagellate blooms, pp 335–344. Ed. by D. L. Taylor and H. H. Seliger. New York: Elsevier/North Holland 1979Google Scholar
  37. Rosenthal, G. A. and D. H. Janzen: Herbivores: their interaction with secondary plant metabolites, 544 pp New York: Academic Press 1979Google Scholar
  38. Scura, E. D. and C. W. Jerde: Various species of phytoplankton as food for larval northern anchovy, Engraulis mordax, and relative nutritional value of the dinoflagellates Gymnodinium splendens and Gonyaulax polyedra. Fish. Bull. U.S. 75, 577–583 (1977)Google Scholar
  39. Sharp, J.: Improved analysis for “particulate” carbon and nitrogen from seawater. Limnol. Oceanogr. 19, 984–989 (1974)Google Scholar
  40. Shimizu, Y.: Recent progress in marine toxin research. Pure appl. Chem. 54, 1973–1980 (1982)Google Scholar
  41. Sokal, R. R. and F. J. Rohlf: Biometry. The principles and practice of statistics in biological research, 2nd ed. 859 pp. San Francisco: W. H. Freeman & Co 1981Google Scholar
  42. Steidinger, K. A.: A re-evaluation of toxic dinoflagellate biology and ecology. In: Progress in phycological research, Vol. 2. pp 147–188. Ed. by F. E. Round and D. J. Chapman. New York: Elsevier 1983Google Scholar
  43. Steidinger, K. A. and D. G. Baden: Toxic marine dinoflagellates. In: Dinoflagellates, pp 201–261. Ed. by D. Spector. New York: Academic Press 1984Google Scholar
  44. Sykes, P. F. and M. E. Huntley: Acute physiological reactions of Calanus pacificus to selected dinoflagellates: direct observations. Mar. Biol. 94, 19–24 (1987)Google Scholar
  45. Venrick, E.: How many cells to count? In: Monographs on oceanographic methodology 6: Phytoplankton manual, pp 167–180. Ed. by A. Sournia. Paris: Unesco Press 1978Google Scholar
  46. Venrick, E.: Percent similarity: the prediction of bias. Fish. Bull. U.S. 81, 375–387 (1983)Google Scholar
  47. Vidal, J.: Physioecology of zooplankton. II. Effects of phytoplankton concentration, temperature, and body size on the development and molting rates of Calanus pacificus and Pseudocalanus sp. Mar. Biol. 56, 135–146 (1980)Google Scholar
  48. White, A. W.: Sensitivity of marine fishes to toxins from the redtide dinoflagellate Gonyaulax excavata and implications for fish kills. Mar. Biol. 65, 255–260 (1981)Google Scholar
  49. White, A. W.: The scope of impact of toxic dinoflagellate blooms on finfish in Canada. Tech. Rep. Fish. aquat. Sciences Can. 1064, 1–5 (1982)Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. E. Huntley
    • 1
  • P. Ciminiello
    • 2
  • M. D. G. Lopez
    • 3
  1. 1.Marine Biology Research Division, Scripps Institution of Oceanography, A-002University of CaliforniaSan Diego; La JollaUSA
  2. 2.Institute of Marine Resources, Scripps Institution of Oceanography, A-028University of CaliforniaSan Diego; La JollaUSA
  3. 3.Scripps Institution of Oceanography, A-008University of CaliforniaSan Diego; La JollaUSA

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