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Isolation of mutants sensitive to 2-amiriopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum

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Summary

Using high performance liquid chromatography, the presence of N6-methyladenine has been found at a level of 0.1 mol percent in DNA extracted from Penicillium chysogenum. No 5-methylcytosine was detected. A mutant strain HP547, which is sensitive to the lethal effects of N-methyl-N1-nitro-N-nitrosoguanidine, methylmethane sulphonate and the base analogue 2-aminopurine shows an increased spontaneous mutation rate and no detectable DNA methylation. Comparison of restriction enzyme digests of wild type and undermethylated strains indicated that methylation was occurring at a different sequence to that of the Dam methylase system of E. coli.

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Author notes

  1. S. D. Rogers & G. Holt

    Present address: The Biological Laboratory, The University of Kent at Canterbury, CT2 7NJ, Canterbury, Kent, UK

Authors and Affiliations

  1. School of Biotechnology, Faculty of Engineering and Science, The Polytechnic of Central London, 115 New Cavendish Street, London, WlM 8JS, UK

    S. D. Rogers, M. E. Rogers, G. Saunders & G. Holt

Authors
  1. S. D. Rogers
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  2. M. E. Rogers
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  3. G. Saunders
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  4. G. Holt
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Rogers, S.D., Rogers, M.E., Saunders, G. et al. Isolation of mutants sensitive to 2-amiriopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum . Curr Genet 10, 557–560 (1986). https://doi.org/10.1007/BF00447390

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  • DOI: https://doi.org/10.1007/BF00447390

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