Archives of Microbiology

, Volume 104, Issue 1, pp 171–178 | Cite as

Electron microscopic characterization of Rhizobium bacteriophage 16-6-12 and its isolated deoxyribonucleic acid

  • Rudi Lurz
  • Frank Mayer
  • Wolfgang Lotz
Article

Abstract

Bacteriophage 16-6-12 of Rhizobium lupini has a long, non-contractile tail and a head which is hexagonal in outline. The tail is 140 nm in length, 11 nm in diameter, and carries a short terminal fiber. Analysis of the tail structure by optical diffraction indicates that it is of the helical “stacked disc” type. After phenol-extraction from purified particles, the DNA of phage 16-6-12 can circularize in vitro. No significant difference in contour length was observed between the linear (14.34±0.28 μm) and circular (14.44±0.24 μm) forms of molecules. After partial denaturation with alkali an AT-GC-map was constructed, which shows an asymmetric distribution of AT- and GC-rich regions. It is concluded that this phage DNA can circularize due to the presence of cohesive ends and that it is not circularly permuted.

Key words

Rhizobium Bacteriophage Electron Microscopy Fine Structure Optical Diffraction DNA “Sticky Ends” Partial Denaturation AT-GC-Map Computer Application 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Rudi Lurz
    • 1
  • Frank Mayer
    • 1
  • Wolfgang Lotz
    • 2
  1. 1.Abt. MikromorphologieInstitut für Mikrobiologie der Universität GöttingenGöttingenGermany
  2. 2.Institut für Mikrobiologie der Universität Erlangen-NürnbergErlangenGermany

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