Advertisement

Archives of Microbiology

, Volume 104, Issue 1, pp 151–154 | Cite as

Conidiation of Neurospora crassa induced by treatment with natrium fluoride in submerged culture

  • W. E. Timberlake
  • G. Turian
Article

Abstract

A transient treatment of pregerminated conidia of Neurospora crassa with NaF induced young, submerged cultures to prematurely differentiate conidia. The inductive treatment decreased the rate of respiration (with lower RQ), reduced the relative concentration of nucleoside triphosphates, and inhibited leucine incorporation into protein and adenosine incorporation into RNA.

Key words

Conidiation Neurospora Natrium Fluoride Submerged 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cortat, M., Turian, G.: Conidiation of Neurospora crassa in submerged culture without mycelial phase. Arch. Microbiol. 95, 305–309 (1974)Google Scholar
  2. Davis, R. H., De Serres, F. J.: Genetic and microbiological research techniques for Neurospora crassa. Meth. Enzymol. 17A, 79–143 (1971)Google Scholar
  3. Emerson, C. P., Humphreys, T.: A simple and sensitive method for quantitative measurement of cellular RNA synthesis. Analyt. Biochem. 40, 254–266 (1971)Google Scholar
  4. Greenberg, D. M.: Metabolic pathways, Vol. 1. New York: Academic Press 1960Google Scholar
  5. Holmsen, H., Holmsen, I., Bernhardsen, A.: Microdetermination of adenosine diphosphate and adenosine triphosphate in plasma with the firefly luciferase system. Analyt. Biochem. 17, 456–473 (1966)Google Scholar
  6. Horowitz, N. H.: Methionine synthesis in Neurospora. The isolation of cystathionine. J. biol. Chem. 171, 255–264 (1947)Google Scholar
  7. Oulevey-Matikian, N, Turian, G.: Contrôle métabolique et aspects ultrastructuraux de la conidiation (macromicroconidies) de Neurospora crassa. Arch. Mikrobiol. 60, 35–58 (1968)Google Scholar
  8. Totten, R. E., Howe, H. B., Jr.: Temperature-dependent actinomycin D effect on RNA synthesis during synchronous development in Neurospora crassa. Biochem. Genetics 5, 521–532 (1971)Google Scholar
  9. Turian, G.: Sporogenesis in Fungi. Ann. Rev. Phytopath. 12, 129–137 (1974)Google Scholar
  10. Turian, G., Bianchi, D. E.: Conidiation in Neurospora. Bot. Rev. 38, 119–154 (1972)Google Scholar
  11. Umbreit, W. W., Burris, R. H. Stauffer, J. F.: Manometric techniques. Minneapolis, Minn.: Burgess 1957Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • W. E. Timberlake
    • 1
  • G. Turian
    • 1
  1. 1.Laboratoire de Microbiologie générale, Département de Biologie végétaleUniversité de GenèveGenève

Personalised recommendations