Abstract
The synthesis of β-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-d-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the presence of the drug. The degree of inhibition is, however, directly related to the 2-deoxy-d-glucose concentration.
References
Farkas, V., Svoboda, A., Bauer, S.: Inhibitory effect of 2-deoxy-d-glucose on the formation of cell wall in yeast protoplasts. J. Bact. 98, 744–748 (1969)
Heredia, C. F., de la Fuente, G., Sols, A.: Metabolic studies with 2-deoxyhexose. I. Mechanisms of inhibition of growth and fermentation in baker's yeast. Biochim. biophys. Acta (Amst.) 86, 216–223 (1964)
Huggett, A. St. G., Nixon, D. A.: Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet 1957II, 368
Johnson, B. F.: Lysis of yeast cell walls induced by 2-deoxy-glucose at their sites of glucan synthesis. J. Bact. 95, 1169–1172 (1968)
Villa, T. G., Notario, V., Villanueva, J. R.: β-Glucanases of the yeast Pichia polymorpha. Arch. Microbiol. 104, 201–206 (1975)
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Villa, T.G., Notario, V., Benítez, T. et al. Synthesis of β-glucanase and other extracellular proteins by cells and protoplasts of the yeast Pichia polymorpha . Arch. Microbiol. 105, 335–337 (1975). https://doi.org/10.1007/BF00447154
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DOI: https://doi.org/10.1007/BF00447154